Process for producing glycosylated indolopyrrolocarbazole derivatives by culturing certain microorganisms

ABSTRACT

A compound of formula (VIII) ##STR1## is added to a culture media containing Microtetraspora sp. A34549 or Saccharothrix aerocolonigenes ATCC 39243. The compound is glycosylated to form an indolopyrrolocarbazole of formula (VII) ##STR2##

CROSS-REFERENCES TO RELATED APPLICATIONS

This application is a continuation of application Ser. No. 08/068,097, filed May 28, 1993 now abandoned, which is a continuation-in-part of Ser. No. 07/981,070, filed Nov. 24, 1992.

BACKGROUND OF THE INVENTION

1. Field of the Invention

This invention is useful in the field of medicine, and relates to novel indolopyrrolocarbazole derivatives inhibiting proliferation of antitumor cells and exhibiting an antitumor effect, a process for preparation thereof and a use thereof.

2. Description of the Related Art

In the field of cancer chemotherapy, many compounds are already put to practical use as an antitumor agent. However, the effect thereof on various kind of tumors is not always adequate, and the problem of resistance of tumor cells against these drugs makes clinical use of these antitumor agents complicated [refer to The 47th Annual Meeting of Japanese Cancer Association Article, pages 12 to 15 (1988)].

In such state of things, development of novel carcinostatic substances is always desired in the field of cancer therapy. Particularly, substances are necessitated which overcome resistance against existing carcinostatic substances and exhibit effectiveness against such kinds of cancers on which existing carcinostatic substances cannot exhibit sufficient effects.

In the light of such present state of things, the present inventors widely screened microbial metabolic products, as a result, found a novel antitumor activity-possessing compound BE-13793C (12,13-dihydro-1,11-dihydroxy-5H-indolo[2,3-a]pyrrolo[3,4-c]carbazole-5,7(6H)dione), and disclosed it [refer to Japanese Laid-Open Patent Publication No. 20277/1991 and J. Antibiotics, 44, 723-728 (1991)].

Thereafter, the present inventors created indolopyrrolocarbazole compounds having an excellent antitumor activity by chemically modifying BE-13793C, and disclosed them (refer to PCT/WO91/18003).

For the purpose of creating compounds having a further excellent antitumor activity by chemically modifying previously disclosed indolopyrrolocarbazole antitumor compounds, the present inventors synthesized many indolopyrrolocarbazole derivatives, investigated their antitumor activity, and as a result, now, found that a series of compounds represented by the following general formula are novel compounds having an extremely excellent antitumor activity.

SUMMARY OF THE INVENTION

Thus, this invention provides indolopyrrolocarbazole derivatives represented by the following general formula and pharmaceutically acceptable salts thereof. ##STR3## wherein R¹ and R² each independently represent a hydrogen atom, lower alkyl group, lower alkenyl group, lower alkynyl group, aryl group, aralkyl group or heterocyclic group (the lower alkyl group, lower alkenyl group, lower alkynyl group, aryl group, aralkyl group and heterocyclic group may each have 1 to 5 substituents selected from the group consisting of carboxyl groups, carbamoyl groups, sulfo groups, amino groups, cyano groups, mono-lower alkylamino groups, di-lower alkylamino groups, hydroxyl groups and halogen atoms), or a group of the formula --Y--R³, and therein Y represents a carbonyl group, thiocarbonyl group or sulfonyl group, and R³ represents a hydrogen atom, lower alkyl group, cycloalkyl group, cycloalkylalkyl group, aryl group, aralkyl group, lower alkoxy group, hydrazino group, amino group, arylamino group, carbamoyl group or heterocyclic group (the lower alkyl group, cycloalkyl group, cycloalkylalkyl group, aryl group, aralkyl group and heterocyclic group may each have 1 to 4 substituents selected from the group consisting of halogen atoms, optionally protected hydroxyl groups, amino groups, carboxyl groups, carbamoyl groups, cyano groups and lower alkoxycarbonyl groups, and the amino group and carbamoyl group may each be mono- or di-substituted by lower alkyl group(s) optionally substituted by substituent(s) selected from the group consisting of halogen atoms, hydroxyl groups, amino groups, carboxyl groups, carbamoyl groups and lower alkoxycarbonyl groups); or

R¹ and R² combine to represent a lower alkylidene group (the lower alkylidene group may have 1 to 4 substituents selected from the group consisting of amino groups, mono-lower alkylamino groups, di-lower alkylamino groups, hydroxyl groups, carboxyl groups and sulfonyl groups); or

R¹ and R² combine together with the nitrogen atom to which they bind to form a heterocyclic group (the heterocyclic group may have on the ring lower alkyl group(s) optionally substituted by group(s) selected from the group consisting of amino groups, hydroxyl groups, a carboxyl groups and sulfo groups),

G represents a pentose group or hexose group, and

X¹ and X² each independently represent a hydrogen atom, halogen atom, amino group, mono-lower alkylamino group, di-lower alkylamino group, hydroxyl group, lower alkoxy group, aralkoxy group, carboxyl group, lower alkoxycarbonyl group or lower alkyl group.

The term of "lower" used in the present invention means that the carbon number of the group or compound to which this term is attached is 6 or less, preferably 4 or less.

DETAILED DESCRIPTION OF THE INVENTION

The "lower alkyl group" is a straight-chain or branched chain alkyl group having 1 to 6 carbon atoms, and examples thereof are a methyl group, an ethyl group, a propyl group, an isopropyl group, a butyl group, an isobutyl group, a sec-butyl group, a tert-butyl group, a pentyl group, an isopentyl group, a neopentyl group, a hexyl group, etc.

The "lower alkenyl group" includes a straight-chain or branched chain alkenyl group having 3 to 6 carbon atoms, and examples thereof are a propenyl group, a 2-butenyl group, a 3-butenyl group, a 3-pentenyl group, a 4-hexenyl group, etc.

The "lower alkynyl group" can be a straight-chain or branched chain alkynyl group having 3 to 6 carbon atoms, and examples thereof are a propynyl group, a 2-butynyl group, a 3-butynyl group, a 3-pentynyl group, a 4-hexynyl group, etc.

The "cycloalkyl group" includes a 3- to 6-membered cycloalkyl group, and examples thereof are a cyclopropyl group, a cyclobutyl group, a cyclopentyl group, a cyclohexyl group, etc.

The "cycloalkyl lower alkyl group" means an alkyl group substituted by a cycloalkyl group wherein the cycloalkyl and lower alkyl parts have the above meanings, respectively, and examples thereof are a cyclopropylmethyl group, a cyclobutylmethyl group, a cyclopentylmethyl group, a cyclohexylmethyl group, a 1-cyclopropylethyl group, a 2-cyclopropylethyl group, a 1-cyclobutylethyl group, a 2-cyclobutylethyl group, a 1-cyclopentylethyl group, a 2-cyclopentylethyl group, a 1-cyclohexylethyl group, a 3-cyclohexylpropyl group, a 3-cyclopentylpropyl group, a 4-cyclohexylbutyl group, a 4-cyclopentylbutyl group, etc., and preferably, the cycloalkylalkyl group has 4 to 10 carbon atoms in total.

The "aryl group" can be monocyclic or polycyclic, and aryl groups having 6 to 12 carbon atoms can be mentioned such as a phenyl group, a naphthyl group and a tetrahydronaphthyl group.

The "aralkyl" group means a lower alkyl group substituted by an aryl group wherein the aryl and lower alkyl parts have the above meanings, respectively, and aralkyl groups having 7 to 15 carbon atoms can be mentioned such as, for example, a benzyl group, a phenethyl group, a phenylpropyl group, a phenylbutyl group, a phenylpentyl group, a naphthylmethyl group and a naphthylethyl group.

The "heterocyclic group" includes a 5- or 6-membered heterocyclic group containing 1 to 4 hetero atoms selected from the group consisting of nitrogen atoms, oxygen atoms and sulfur atoms, and there can be mentioned aromatic heterocyclic groups such as, for example, a pyrrolyl group, a furyl group, a thienyl group, an oxazolyl group, an isoxazolyl group, a thiazolyl group, an isothiazolyl group, an imidazolyl group, a pyrazolyl group, an oxadiazolyl group, a thiadiazolyl group, a triazolyl group, a tetrazolyl group, a furazanyl group, a pyridyl group, a pyridazinyl group, a pyrimidinyl group, a pyrazinyl group and a triazinyl group; and nonaromatic heterocyclic groups such as, for example, a dihydrothienyl group, a tetrahydrothienyl group, a pyrrolinyl group, a pyrrolidinyl group, an imidazolidinyl group, an imidazolinyl group, a piperidinyl group, a piperazinyl group, an oxazolinyl group, an oxazolidinyl group, an isoxazolinyl group, an isoxazolidinyl group, a thiazolinyl group, a thiazolidinyl group, an isothiazolinyl group, an isothiazolidinyl group, a 1,2-dithiolanyl group, a 1,3-dithiolanyl group, a 1,2-dithiolyl, a 1,3-dithiolyl group, a dihydrothiopyranyl group, a tetrahydrothiopyranyl group, a 1,4-dithianyl group, a 1,4-dithiinyl group, a 1,4-oxathiinyl group and a thiomorpholinyl group.

As the "mono-lower alkylamino groups", there can, for example, be mentioned a methylamino group, an ethylamino group, a propylamino group, an isopropylamino group, a butylamino group, a pentylamino group, a hexylamino group, etc., and as the "di-lower alkylamino groups" there can, for example, be mentioned a dimethylamino group, an ethylmethylamino group, a diethylamino group, an ethylpropylamino group, a dipropylamino group, a butylmethylamino group, a dibutylamino group, a butylethylamino group, a methylpentylamino group, a hexylmethylamino group, an ethylhexylamino group, etc.

The "arylamino group" means an amino group substituted by an aryl group wherein the aryl part has the above meanings, and the arylamino group can be mentioned such as, for example, a phenylamino group and a naphthylamino group.

The "halogen atoms" include a fluorine atom, a chlorine atom, a bromine atom and an iodine atom.

As the "lower alkylidene groups", there can be mentioned straight-chain or branched alkylidene groups having 1 to 6 carbon atoms such as, for example, a methylene group, an ethylidene group, a propylidene group, an isopropylidene group, a butylidene group, an isobutylidene group, a sec-butylidene group, a pentylidene group, an isopentylidene group, a neopentylidene group and a hexylidene group.

The "lower alkoxy group" means a (lower alkyl)-O-group wherein the lower alkyl part has the above meaning, and examples thereof are a methoxy group, an ethoxy group, a propoxy group, an isopropoxy group, a butoxy group, an isobutoxy group, a sec-butoxy group, a tert-butoxy group, a pentoxy group, an isopentoxy group, a neopentoxy group, a hexoxy group, etc.

The "lower alkyloxycarbonyl group" means a (lower alkoxy)-CO-group wherein the lower alkoxy part has the above meaning, and examples thereof are a methoxycarbonyl group, an ethoxycarbonyl group, a propyloxycarbonyl group, an isopropyloxycarbonyl group, a butyloxycarbonyl group, an isobutyloxycarbonyl group, a pentyloxycarbonyl group, a hexyloxycarbonyl group, etc.

The "aralkoxy group" means a lower alkoxy group substituted by an aryl group wherein the aryl and lower alkoxy parts have the aforesaid meanings, respectively, and examples thereof are a benzyloxy group, a phenethyloxy group, a phenylpropoxy group, an α-naphthylmethoxy group, a β-naphthylmethoxy group, a naphthylethoxy group, a tetrahydronaphthylmethoxy group, etc.

Mentioned as examples of the protective group in the "optionally substituted hydroxyl group" are alkanoyl groups having 2 to 6 carbon atoms such as an acetyl group, a propionyl group and a butyryl group; aroyl groups such as a benzoyl group; substituted or unsubstituted aralkyl groups such as a benzyl group and a 4-methoxybenzyl group; groups forming an acetal such as acetonide; etc.

The "pentose group" and "hexose group" mean a pentose group and a hexose group the hydroxyl groups of which may be substituted by the same or different 1 to 3 groups selected from the group consisting of hydrogen atoms, lower alkyl groups, lower alkylcarbonyloxy groups, lower alkoxy groups and amino groups, or oxidized, and there can be mentioned groups derived from pentoses such as, for example, ribose, arabinose, xylose and 2-deoxyribose, and groups derived from hexoses such as, for example, allose, glucose, mannose, galactose, glucosamine, galactosamine, 2-deoxyglucose, 4-O -methylglucose, rhamnose and glucuronic acid.

Preferred among the compounds of the aforesaid formula [I] provided by this invention are compounds represented by the following formula ##STR4## wherein R¹¹ and R²¹ each independently represent a hydrogen atom, lower alkyl group, lower alkenyl group, aryl group, aralkyl group, pyrrolyl group, oxazolyl group, isoxazolyl group, thiazolyl group, imidazolyl group, pyridyl group, pyrimidinyl group, oxazolinyl group, oxazolidinyl group, imidazolinyl group, imidazolidinyl group, pyrrolidinyl group, piperazinyl group, thiazinyl group, thiazolidinyl group (the lower alkyl group, lower alkenyl group, aryl group, aralkyl group and heterocyclic group may have 1 to 5 substituents selected from the group consisting of carboxyl groups, carbamoyl groups, cyano groups and hydroxyl groups), or a group of the formula --Y--R³¹ and therein y represents a carbonyl group, thiocarbonyl group or sulfonyl group, and R³¹ represents a hydrogen atom, lower alkyl group, aryl group (the lower alkyl group and aryl group may have 1 to 4 substituents selected from the group consisting of halogen atoms, optionally protected hydroxyl groups, amino groups and carboxyl groups), amino group, hydrazino group, arylamino group, lower alkoxy group, carbamoyl group, pyrrolyl group, oxazolyl group, isoxazolyl group, thiazolyl group, imidazolyl group, pyridyl group, pyrimidinyl group, oxazolinyl group, oxazolidinyl group, imidazolinyl group, imidazolidinyl group, pyrrolidinyl group, piperazinyl group, thiazinyl group or thiazolidinyl group; or

R¹¹ and R²¹ combine to represent a lower alkylidene group optionally having carboxyl group(s), or

R¹¹ and R²¹ combine together with the nitrogen atom to which they bind to form a pyrrolidinyl group, imidazolidinyl group, imidazolinyl group, piperidino group or, pieprazinyl group (these heterocyclic groups may have on the ring lower alkyl group(s) optionally substituted by hydroxy group(s)),

G¹ represents a group of the formula ##STR5## and therein R⁷ represents a hydrogen atom or lower alkyl group and R⁸ represents a hydroxyl group or amino group, and

X¹¹ and X²¹ bind to the indolopyrrolocarbazole rings at the 1- or 2-position and at the 10- or 11-position, respectively, and each independently represent a halogen atom, hydroxyl group, lower alkoxy group or aralkoxy group.

Further preferred compounds are those represented by the following formula ##STR6## wherein R¹² represents a hydrogen atom or lower alkyl group,

R²² represents a hydrogen atom, lower alkyl group (the lower alkyl group may have 1 to 5 substituents selected from the group consisting of carboxyl groups, carbamoyl groups, hydroxyl groups and cyano groups), aryl group, aralkyl group (the aryl group and aralkyl group may have 1 to 4 substituents selected from the group consisting of hydroxyl groups and carboxyl groups), pyridyl group, imidazolyl group, imidazolinyl group, thiazolyl group, pyrrolidinyl group, piperazinyl group, or a group of the formula --Y--R³² and therein y represents a carbonyl group, thiocarbonyl group or sulfonyl group, and when y is a carbonyl group or thiocarbonyl group, R³² represents a hydrogen atom, lower alkyl group, aryl group (the lower alkyl group and aryl group may have 1 to 4 substituents selected from the group consisting of halogen atoms, optionally protected hydroxyl groups, amino groups and carboxyl groups), amino group, hydrazino group, arylamino group, lower alkoxy group, carbamoyl group, pyridyl group, pyrimidinyl group, imidazolinyl group or pyrrolidinyl group, and when y is a sulfonyl group, R³² represents a lower alkyl group or aryl group; or

R¹² and R²² combine to represent a lower alkylidene group having carboxyl group(s); or

R¹² and R²² combine together with the nitrogen atom to which they bind to form a pyrrolidinyl group, piperidino group or piperazinyl group (these heterocyclic groups may have on the ring lower alkyl group(s) optionally having hydroxyl group(s)), and

G¹, X¹¹ and X²¹ have the same meanings as defined in the above formula [Ia].

Preferred as G¹ is generally ##STR7## and preferred as X¹¹ and X²¹ are hydroxyl groups bound to the 1-position and 11-position of the indolopyrrolocarbazole ring, respectively.

The compounds of this invention can exist in the form of pharmaceutical acceptable salts. Such salts include addition salts with inorganic acids such as, for example, hydrochloric acid and sulfuric acid, and with organic acids such as, for example, acetic acid, citric acid, tartaric acid and maleic acid. Further, in case the compounds of this invention contain an acidic group, the acidic group can exist in the form of alkali metal salts such as, for example, a potassium salt and a sodium salt; alkaline earth metal salts such as, for example, a magnesium salt and a calcium salt; and salts with organic bases such as an ethylamine salt and an arginine salt.

A compound of the formula [I] set forth in this invention can be prepared by reacting a compound represented by the following formula or a derivative thereof wherein the functional groups are protected ##STR8## wherein, Y represents a hydrogen atom or substituted or unsubstituted lower alkyl group, and X¹, X² and G have the same meanings as defined above with a compound represented by the following general formula or a derivative thereof wherein in case R¹³ and R²³ contain a functional group, the functional group is each protected ##STR9## wherein R¹³ and R²³ each independently represent a hydrogen atom, lower alkyl group, lower alkenyl group, lower alkynyl group, aryl group, aralkyl group or heterocyclic group (the lower alkyl group, lower alkenyl group, lower alkynyl group, aryl group, aralkyl group and heterocyclic group may have 1 to 5 substituents selected from the group consisting of carboxyl groups, carbamoyl groups, sulfo groups, amino groups, cyano groups, mono-lower alkylamino groups, di-lower alkylamino groups, hydroxyl groups and halogen atoms), or a group of the formula --Y--R³ and herein y represents a carbonyl group, thiocarbonyl group or sulfonyl group, and R³ represents a hydrogen atom, lower alkyl group, cycloalkyl group, cycloalkylalkyl group, aryl group, aralkyl group, lower alkoxy group, hydrazino group, amino group, arylamino group, or carbamoyl group or heterocyclic group (the lower alkyl group, cycloalkyl group, cycloalkylalkyl group, aryl group, aralkyl group and heterocyclic group may each have 1 to 4 substituents selected from the group consisting of halogen atoms, optionally protected hydroxyl groups, amino groups, carboxyl groups, carbamoyl groups, cyano groups and lower alkoxycarbonyl groups, and the amino group and carbamoyl group may each be mono- or di-substituted by lower alkyl group(s) optionally substituted by group(s) selected from the group consisting of halogen atoms, hydroxyl groups, amino groups, carboxyl groups, carbamoyl groups and lower alkoxycarbonyl groups); or

R¹³ and R²³ combine together with the nitrogen atom to which they bind to form a heterocyclic group (the heterocyclic group may have on the ring lower alkyl group(s) optionally substituted by group(s) selected from the group consisting of amino groups, hydroxyl groups, carboxyl groups and sulfo groups); if necessary, removing the protective group(s) existing in the product to prepare a compound represented by the general formula ##STR10## wherein R¹³, R²³, X¹, X², G have the same meanings as defined above;

or either formylating, alkylating, alkenylating, alkynylating, aralkylating, carbamoylating, thiocarbamoylating, alkanoylating or sulfonylating the amino group ##STR11## of the compound of the above formula [Ic] or the derivative thereof wherein the functional groups are protected when R¹³ and R²³ represent a hydrogen atom, or condensing the above compound or derivative with a compound represented by the following formula or a derivative thereof wherein a functional group is protected

    OHC--R.sup.6                                               [v]

wherein R⁶ represents a hydrogen atom or carboxyl group, or a lower alkyl group optionally having 1 to 4 substituents selected from the group consisting of amino groups, mono- lower alkylamino groups, di-lower alkylamino groups, hydroxyl groups, carboxyl groups and sulfo groups,

and if necessary, removing the protective groups existing in the product; or reducing the double bonds of the compound of the above formula [Ic] when R¹³ and/or R²³ contain a double bond or the compound prepared by condensing the compound [Ic] and the compound [IV], or the derivative thereof wherein the functional groups are protected, and if necessary removing the protective groups existing in the product; and if necessary, converting the resulting compound of the formula [I] into a pharmaceutically acceptable salt.

Herein, the terms of alkylation, alkenylation, alkynylation, aralkylation, alkanoylation and sulfonylation are widely interpreted, and mean all of the reactions to introduce substituents corresponding to R¹ and R² in the structure of the compounds of this invention, and for example, alkylation means introduction of a substituted or unsubstituted alkyl group included in this invention.

Reaction of a compound of the formula [II] or [III] (hereafter, including a derivative thereof wherein a functional group is introduced) with a compound of the formula [IV] (hereafter, including a derivative thereof wherein its functional groups are protected) can be carried out in accordance with reaction known per se of an imide or acid anhydride with a hydrazine or a hydrazine derivative, and can, for example, be carried out in the absence of a solvent or in an inert solvent, for example a solvent such as N,N-dimethylformamide at a temperature between about 0° C. and the boiling point of the solvent, preferably in the range of about room temperature to about 80° C.

The use quantity of the compound of the formula [IV] to the compound of the formula [II] or [III] is not particularly limited, and can be varied over a wide range according to the kind of the compound, reaction conditions, etc., but usually, it is suitable to use the compound of the formula [IV] in a quantity in the range of at least 1 mole, preferably 1 to 10 moles, particularly 3 to 5 moles per mole of the compound of the formula [II] or [III]. Further, when the compound of the formula [IV] is liquid in the reaction temperature, it is also possible to use the compound in a largely excessive quantity, for example in a rate of 10 to 40 moles per mole of the compound of the formula [II] or [III] so as to make it serve as a solvent.

Thereby, there can be obtained a compound of the above formula [Ic]0 wherein the existing functional groups are sometimes protected appropriately.

The thus obtained compound of the formula [Ic] in case R¹³ and R²³ represent a hydrogen atom or a derivative thereof wherein its functional groups are protected (hereafter, generally referred to as a compound of [Ic-1]) can be formylated, alkylated, alkenylated, alkynylated, aralkylated, carbamoylated, thiocarbamoylated, alkanoylated or sulfonylated to give a corresponding compound of the formula [Ic] in case at least one of R¹³ and R²³ represents a group except for a hydrogen atom defined on these groups.

Formylation of a compound of the formula [Ic-1] can be carried out according to a method usually used in formylation of an amino group, and can, for example, be carried out by heating it together with formic acid, formamide, dimethylformamide or the like, or by a method to react it with a mixture of formic acid and an acid anhydride in a solvent having no bad influence or without any solvent, or by another means.

Reaction of the compound of the formula [Ic-1] with formic acid, formamide, dimethylformamide or the like is usually carried out at a temperature in the range of 30° C. to the boiling point of the solvent, but if necessary, can also be carried out at a temperature above or under such temperature, and reaction time is usually in the range of 30 minutes to 2 days. Preferably, the reaction is carried out usually in the presence of an acid catalyst such as hydrochloric acid or sulfuric acid.

Formylation using a mixture of formic acid with an acid anhydride is usually carried out at a comparatively low temperature in the range of -5° C. to room temperature, but can, if necessary, be carried out in a range above or under this. Further, reaction time is usually 10 minutes to 5 hours, but can, if necessary, be lengthened or shortened.

Alkylation, alkenylation, alkynylation and aralkylation of a compound of the formula [Ic-1] can be carried out in accordance with a method known per se, for example, reaction with an alkylating agent, alkenylating agent, alkynylating agent or aralkylating agent such as an alkyl halide, an alkenyl halide, an alkynyl halide, an aralkyl halide, an alkyl mesylate, an alkenyl mesylate, an aralkyl mesylate, an alkyl tosylate or an aralkyl tosylate; or a method to condense it with an aldehyde compound or a ketone compound and reduce the resultant condensate; or the like. The reduction reaction at that time can be carried out according to a method using formic acid, a metal or a metal hydride or a usual method such as a catalytic reduction method using palladium-carbon or the like.

Carbamoylation and thiocarbamoylation of a compound of the formula [Ic-1] can be carried out by reacting it with a correspond isocyanate compound or thioisocyanate compound in the absence of solvent or in a suitable solvent. Reaction temperature can be in the range of about -20° C. to the boiling point of the solvent, preferably about 0° to about 50° C.

Alkanoylation of a compound of the formula [Ic-1] can be carried out by a method to react it with a corresponding acid halide or an acid anhydride in the absence of a solvent or in a suitable solvent. Reaction can usually be carried out at a temperature in the range of about -5° C. to the boiling point of the solvent, and if necessary, can also be carried out at a temperature below this.

The acid halide or acid anhydride is, usually, used in a rate of small excess to the compound of the formula [Ic-1], but can, if necessary, be used in a quantity below or above this, and reaction time can, usually, be 30 minutes to 2 days.

Sulfonylation of a compound of the formula [Ic-1] can be carried out by reacting it with a reagent such as a corresponding organic sulfonic acid anhydride or organic sulfonyl halide in the presence or absence of a base. Reaction temperature can, usually, be sufficient in the range of about -10° C. to about 50° C., but can, if necessary, be a temperature above or under this, and reaction time can, usually, be 30 minutes to 3 days. A reagent such as an organic sulfonic acid anhydride or an organic sulfonyl halide is, usually, used in a rate of small excess, but can also be used, in a quantity above or under this.

Further, condensation reaction of a compound of the formula [Ic-1] with a compound of the above formula [V] (including a derivative thereof wherein the functional groups are protected) is so-called Schiff base formation reaction, and can, for example, usually be carried out in a solvent inert to the reaction, e.g. in a solvent such as tetrahydrofuran, at a temperature between about 0° C. to the boiling point of the solvent, preferably in the range of room temperature to about 50° C. Reaction time is usually in the range of 30 minutes to 2 days, but can, if necessary, be a time above or under this.

Use quantity of the compound of the formula [V] to the compound of the formula [Ic-1] is not strictly limited, but usually, it is suitable to use the compound of the formula [V] in a rate of 1 to 50 moles, particularly 3 to 10 moles per mole of the compound of the formula [Ic-1].

The hydrazone compound obtained by the above reaction can be subjected to usual catalytic hydrogenation reaction using palladium-carbon or the like to give a compound of the formula [I] wherein R¹ or R² represents a hydrogen atom.

In the foregoing processes, protection of functional groups in raw material compounds and removal of protective groups existing in the formed compounds can be carried out using usual and optional methods widely known in the chemical field.

Further, isolation and purification of compounds produced by the above reactions can be carried out according to methods known per se in the field of organic synthetic chemistry, for example precipitation methods, solvent extraction methods, recrystallization, chromatography, etc.

A compound of the above formula [II] used as a starting raw material in the aforesaid processes can be prepared by glycosylating a compound represented by the general formula ##STR12## wherein X¹, X² and Y have the same meanings as defined above prepared by a process known per se [refer to J. Chem. Soc. Perkin Transactions I. pp 2475-2480 (1990)] or a derivative thereof wherein the functional groups are protected.

Glycosylation of the compound of the formula [VI] or the derivative thereof wherein the functional groups are protected can be carried out by a process known per se [refer to J. Am. Chem. Soc. 60, 2559 (1938)], for example by condensing it with a reactive derivative of a pentose or hexose wherein the hydroxyl groups are protected, e.g. 1-bromo-2,3,4,6-O-tetraacetylglucose, using as an activating agent mercury cyanide, silver carbonate, silver oxide or the like, preferably silver oxide, in an aprotic solvent, e.g. a solvent such as benzene, toluene or methylene chloride at a temperature of about 0° C. to about 100° C., preferably about 80° C.

Alternatively, a compound of the formula [II] can also be prepared according to the process disclosed in the aforesaid PCT/WO91/18003.

Further, a compound of the formula [III] can be prepared by treating with a base a thus obtained compound of the formula [II] or derivative thereof wherein the functional groups are protected.

Preferred as the base is an aqueous solution of potassium hydroxide, and treatment with this base can usually be carried out at room temperature, but in some occasions, can also be carried out with heating up to a temperature of about 50° C.

Neutralization or acidification of the reaction mixture can, if necessary, be carried out using hydrochloric acid, and thereby it is possible to precipitate the compound of the formula [III] as crystals.

Further, a compound represented by the general formula ##STR13## wherein X¹ and X² have the same meanings as defined above, which is used as a starting raw material in the aforesaid process and is included in the formula [II], can be prepared by culturing a microorganism having an ability to glycosylate a compound represented by the general formula ##STR14## wherein X¹ and X² have the same meanings as defined above, in a nutrient medium, and recovering the compound represented by the general formula [VII] above from the culture medium.

As a glycosyl group represented by the formula ##STR15## there can, for example, be mentioned as allose, altrose, glucose, mannose, gulose, idose, galactose or talose residue, and a glucose residue is particularly preferable.

Any microorganism can be used in the above production process so long as it is a microorganism capable of glycosylating a compound [VIII] and for example, there can be used Microtetraspora sp. A34549 strain, a kind of Actinomyces isolated from the soil of Yoro Keikoku, Ichihara-shi, Chiba-ken, Saccharothrix aerocolonigenes ATCC 39243 strain, or the like.

Bacteriological characteristics of Microtetraspora sp. A 34549 strain are shown below:

1. Morphological properties

The A 34549 strain does not form genuine aerial mycelia, and no spores are observed on the substrate mycelia. Further, division of mycelia is not observed, and specific organs such as sporangia and sclerotia are not observed.

2. Characteristics on culture on various agar plate media.

The results of culture at 28° C. for 14 hours in various agar plate media are shown in Table 1.

                  TABLE 1                                                          ______________________________________                                                                       Color of                                                             Aerial    substrate                                                                             Soluble                                   Medium     Growth   mycelium  mycelium                                                                              pigment                                   ______________________________________                                         Yeast maltose                                                                             good     trace     yellowish                                                                             brownish                                  agar (ISP-2)                  brown  orange                                    Oatmeal agar                                                                              good     not       grayish                                                                               none                                      (ISP-3)             formed    brown                                            Starch     poor     not       colorless                                                                             none                                      inorganic salt      formed                                                     agar (ISP-4)                                                                   Glycerol   scarcely                                                            asparagine grow                                                                agar (ISP-5)                                                                   Peptone yeast                                                                             good     not       yellowish                                                                             none                                      iron agar           formed    brown                                            (ISP-6)                                                                        Tyrosine agar                                                                             scarcely                                                            (ISP-7)    grow                                                                Nutrient agar                                                                             good     not       bright light                                                         fomred    yellowish                                                                             yellow                                                                  brown                                            Sucrose    scarcely                                                            nitrate agar                                                                              grow                                                                Glucose    scarcely                                                            asparagine grow                                                                agar                                                                           ______________________________________                                    

3. Growth temperature (yeast maltose agar medium, culture for 14 days) Grows at 18° C.-43° C. and optimum growth

temperature seems to be 30° C. or so.

4. Physiological properties

(1) Liquefaction of gelatin positive (weak) (glucose peptone gelatin medium)

(2) Hydrolysis of starch negative (starch inorganic salt medium)

(3) Coagulation of skim milk powder negative (skim milk medium)

(4) Peptonization of skim milk powder positive (weak) (skim milk medium)

(5) Formation of melanoid pigment negative

(6) Common salt resistance grows at common (yeast maltose agar medium) salt content of 2% or less

5. Ability to utilize carbon sources

Culture was carried out at 28° C. for 14 days using Pridham-Godleave agar as a basal medium and adding the following various sugars. As a result, no growth was observed and judgment could not been made.

D-glucose, D-xylose, L-arabinose, L-rhamnose,

D-fructose, D-galactose, raffinose, D-mannitol, inositol, salicin, sucrose

6. Cell components

From the cell wall meso-diaminopimelic acid was detected but glycine, arabinose and galactose were not detected, and it was suggested that the type of the cell wall was type III. As the main sugar components of all the cells, madulose (arabinose, galactose and xylose are not contained) was detected, and its sugar pattern was type B, its phospholipid type was PIV and the main component of menaquinone was MK-9 (H4).

From the above microbilogical properties, the A strain was considered to belong to the genus Microtetraspora, an Actinomyces, and the A34549 strain was designated Microtetraspora sp. A34549. This strain was deposited on Nov. 17, 1992 with the Fermentation Research Institute, Agency of Industrial Science & Technology, at 1-3, Higashi 1 chome Tsukuba-shi Ibaraki-ken 305 JAPAN, and the accession number is FERM P-13292, and after conversion to deposition under Budapest treaty as of Feb. 25, 1993, FERM BP-4206.

A mutant of a microorganism having a glycosylating ability can be obtained by mutating a microorganism having an ability to glycosylate the compound represented by the general formula [VIII] and to convert it to the compound represented by the general formula [VII], by a method usually used for a strain transformation treatment, for example, an irradiation treatment such as, e.g., X-ray or ultraviolet ray application; a treatment with a mutagen such as, e.g., nitrogen mustard, azaserine, nitrous acid, 2-aminopurine or N-methyl-N'-nitro-N-nitrosoguanidine (NTG); a phase contact transformation, transduction, conjugation, or the like.

A microbial strain used in the conversion of the compound of the formula [VIII] to the compound of the formula [VII] is inoculated in a nutrient--containing medium and grown aerobically. As nutrient sources, those known as nutrient sources for microorganisms can be used. For example, as carbon sources, there can be used solely or as a mixture glucose, allose, altrose, mannose, gulose, idose, galactose, talose, glycerol, maltose, starch, sucrose, molasses, dextrin, etc. placed on the market. As nitrogen sources, there can be used solely or as a mixture soybean meal, corn gluten meal, corn steep liquor, meat extract, yeast extract, cotton seed meal, peptone, wheat germ, fish meal, inorganic ammonium salts, sodium nitate, etc. placed on the market. As inorganic salts, there can be used calcium carbonate, sodium chloride, potassium chloride, magnesium sulfate, various phosphate salts, etc. Further, if necessary, salts of heavy metals such as iron, manganese, molybdenum, copper and zinc can be added in a trace quantity. Further, when there is too much foaming, there can appropriately, for example, be added as an antifoaming agent a vegetable oil such as soybean oil or linseed oil, a higher alcohol such as octadecanol, a silicone compound, or the like. Besides the above materials, there can be used any of materials utilized by the microorganism and useful for its growth such as, for example, 3-(N-morpholino) propanesulfonic acid or sodium borate.

As for a culture process, culture can be carried out in the same manner as in a general process for production of microbial metabolites except for addition of the compound [VIII], and either solid culture or liquid culture can be adopted. In case of liquid culture, any of standing culture, stirring culture, shaking culture, aeration culture, etc. can be utilized, but shaking culture or submerged aeration stirring culture is preferable. Culture temperature is suitably 20° to 37° C., preferably 25° to 30° C. Preferred pH of a medium is in the range of 4 to 8, and a culture term is 2 to 20 days, preferably 7 to 15 days

For recovery of the desired compound of the formula [VII] from the culture medium, there can appropriately be utilized separation means usually used for recovery of metabolites produced by microorganisms from their culture media. Since the compound [VII] exists in the culture filtrate or in the cells, it can be purified from the culture filtrate or the cells according to usual separation means such as, for example, solvent extraction method, ion exchange resin method, adsorption or partition chromatography, or gel filtration, either alone or in combination. Further, high performance liquid chromatography, thin layer chromatography or the like can also be utilized for extraction and purification.

The compounds of the formula [I] provided by this invention have an excellent antitumor action as shown in the following pharmacological test examples.

(1) Therapeutic effect against mouse tumor (P388)

Therapeutic effect of the compounds of this invention against mouse tumor (P388 ) is shown in Tables 2 and 3.

                  TABLE 2                                                          ______________________________________                                         Effect of the compound of Example 2 against P388                                       Dose.sup.(2), i.p.                                                     Tumor.sup.(1)                                                                          (mg/kg/injection)                                                                           MST.sup.(3) (day)                                                                           T/C (%).sup.(4)                              ______________________________________                                         P388    0            12.3 ± 1.06                                                                              100                                                  1            15.8 ± 0.84                                                                              128                                                  3            17.8 ± 1.92                                                                              145                                                  10           >26.4 ± 18.82                                                                            >245                                                 30           >42.2 ± 24.38                                                                            >343                                                 100          >47.2 ± 18.90                                                                            >384                                         ______________________________________                                    

                  TABLE 3                                                          ______________________________________                                         Effect of the compound of Example 5 against P388                                       Dose.sup.(2), i.p.                                                     Tumor.sup.(1)                                                                          (mg/kg/injection)                                                                           MST.sup.(3) (day)                                                                           T/C (%).sup.(4)                              ______________________________________                                         P388    0            12.3 ± 0.95                                                                              100                                                  1            16.8 ± 0.84                                                                              137                                                  3            17.8 ± 1.92                                                                              145                                                  10           >26.2 ± 10.18                                                                            >213                                                 30           >23.4 ± 9.74                                                                             >190                                                 100          >36.4 ± 8.05                                                                             >296                                         ______________________________________                                          (Footnotes of Tables 2 and 3)                                                  .sup.(1) Tumor inoculation: 10.sup.6 cancer cells were intraperitoneally       inoculated.                                                                    .sup.(2) Dose: After tumor inoculations, each dose was intraperitoneally       administered once a day from the 1st day to 10th day.                          .sup.(3) MST: mean survival time (day)                                         .sup.(4) T/C (%): (MST of treatment group/MST of control group) ×        100                                                                            .sup.(5) Standard: in case of T/C ≧ 125, the test compound was          judged to have a remarkable antitumor effect in the dose.                

(2) Proliferation inhibition activity against mouse leukemia cell

Measurement method:

100 μl portions of a cell culturing medium (10% fetal bovine serum-containing-RPMI-1640 medium) containing 3×10³ mouse leukemia cell (P388) were put in a 96-hole microplate, the cells were cultured under 5% CO₂ at 37° C. for 24 hours, 10 μl each of test solutions containing test compounds were added respectively, and the cells were further cultured under 5% CO₂ at 37° C. for 24 hours. 10 μl portions of 0.5% Thiazoyl Blue were added to the culture medium, and incubation was carried out under 5% CO₂ at 37° C. for 2 hours to carry out enzymatic reaction. 20% sodium dodecyl sulfate (SDS) was added to discontinue the reaction, incubation was further carried out at 37° C. for 4 hours to dissolve the formed dye, and absorbance at 550 nm was measured and compared with the control group. The results are shown in Table 4.

                  TABLE 4                                                          ______________________________________                                         Proliferation inhibition activity against mouse                                leukemia cell P388                                                                              50% inhibitory concentration                                  Test compound    (IC.sub.50, μM)                                            ______________________________________                                         Compound of Example 1                                                                           <0.030                                                        Compound of Example 2                                                                           0.29                                                          Compound of Example 3                                                                           0.065                                                         Compound of Example 4                                                                           0.096                                                         Compound of Example 5                                                                           0.28                                                          Compound of Example 6                                                                           0.059                                                         Compound of Example 7                                                                           0.091                                                         Compound of Example 8                                                                           0.30                                                          Compound of Example 9                                                                           0.028                                                         Compound of Example 10                                                                          0.46                                                          Compound of Example 11                                                                          <0.026                                                        Compound of Example 12                                                                          0.042                                                         Compound of Example 13                                                                          0.22                                                          Compound of Example 14                                                                          <0.027                                                        Compound of Example 15                                                                          0.31                                                          Compound of Example 17                                                                          0.044                                                         Compound of Example 22                                                                          0.11                                                          Compound of Example 23                                                                          <0.025                                                        Compound of Example 24                                                                          0.001                                                         Compound of Example 25                                                                          0.048                                                         Compound of Example 27                                                                          0.027                                                         Compound of Example 28                                                                          <0.029                                                        Compound of Example 29                                                                          0.005                                                         Compound of Example 30                                                                          0.003                                                         Compound of Example 31                                                                          0.011                                                         Compound of Example 33                                                                          0.11                                                          Compound of Example 34                                                                          0.019                                                         Compound of Example 35                                                                          0.17                                                          Compound of Example 36                                                                          0.002                                                         Compound of Example 37                                                                          0.095                                                         ______________________________________                                    

As apparent from the results of the above pharmacological test, the compounds of this invention exhibit an excellent antitumor action, and are useful as an antitumor agent for control or prevention of diseases, particularly for treatment of cancers. When a compound of this invention is used in such uses, it is, usually, formulated into a pharmaceutical preparation comprising an effective quantity of it and a pharmaceutically acceptable carrier or diluent.

As administration forms at the time of use of a compound of this invention, various forms can be selected, and there can be mentioned oral agents such as, for example, tablet, capsuls, powders, granules or liquids, or sterilized liquid parenteral agents such as, for example solutions or suspensions, or suppositories, ointments, or the like.

Solid preparations can be prepared, as they are, as forms of tablets, capsuls, granules or powders, or can also be prepared using suitable additives. Such additives may be additives usually used, and include saccharides such as, for example, lactose and glucose; starches such as, for example, corn, wheat and rice; fatty acids such as, for example, stearic acid; inorganic salts such as, for example, magnesium metasilicate aluminate and anhydrous calcium phosphate; synthesized macro-molecules such as, for example, polyvinylpyrrolidone and polyalkylene glycol; fatty acid salts such as, for example, calcium stearate and magnesium stearate; alcohols such as, for example, stearyl alcohol and benzyl alcohol; synthesized cellulose derivatives such as, for example, methylcellulose, carboxymethylcellulose, ethylcellulose and hydroxypropylmethylcellulose; and further, gelatin, talc, vegetable oils, gum arabic, etc.

Solid preparations such as these tablets, capsuls, granules and powders contain an effective ingredient generally at 0.1-100 weight %, preferably at 5-100 weight %.

Liquid preparations are prepared in forms such as suspensions, syrups, injections or drops using suitable additives usually used in liquid preparations such as water, alcohols or oils originated in vegetables such as, for example, soybean oil, peanut oil and sesame oil.

Particularly, solvents suitable in case of parenteral administration in the form of intramuscular injection, intravenous injection or subcutaneous injection include, for example, distilled water for injection, aqueous lidocaine hydrochloride solutions (for intramuscular injection), physiological saline, aqueous glucose solutions, ethanol, polyethylene glycol, liquids for intravenous injection (e.g. aqueous solutions of citric acid and sodium citrate, etc.), electrolyte solutions (for intravenous drip and intravenous injection), etc., and their mixed solvents.

These injections can take forms that powder itself or to which suitable additives were added is dissolved at the time of use, besides such forms that ingredients are dissolved in advance. Such an injection contains usually 0.1-10 weight %, preferably 1-5 weight % of the effective ingredient.

Further, a liquid agent of a suspension, syrup or the like for oral administration can usually contain 0.5-10 weight % of the effective ingredient.

The preferred dose of the compounds of this invention can be varied according to the kind of a compound to be used, the kind and application frequency of the compounded composition, the specified site to be treated, the degree of diseases, the age of patients, diagnosis of doctors, the kind of tumor, etc., but, as an approximate standard, the dose per day and per one adult can, for example, be in the range of 10 to 1000 mg in case of oral administration, and in the range of 10 to 500 mg in case of parenteral administration, preferably intravenous injection. The administration frequency varies depending on administration methods and symptoms, but is 1 to 5 times a day. Further, there can also be adopted administration methods such as intermittent administration, e.g. every second day administration or every third day administration.

EXAMPLES

This invention is more specifically described below by examples, but not limited only by these examples.

Example A

The compound represented by the formula ##STR16##

3.4 g of 12,13-dihydro-1,11-dihydroxy-13-(β-D-glucopyranosyl)-5H-indolo[2,3-a]pyrrolo[3,4-c]carbazole-5,7(6H)-dione was dissolved in 120 ml of 10% aqueous potassium hydroxide solution, and the solution was stirred at room temperature for 2 hours. The reaction solution was neutralized with addition of 120 ml of 2N hydrochloric acid, and the precipitated red crystals were filtered, washed with water and dried to give 3.0 g of the captioned compound.

FAB-MS(m/z): 520 (M)⁺, 521 (M+H)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 3.42 (1H,m), 3.56-3.70 (2H,m), 3.76 (1H,m), 3.95-4.10 (2H,m), 4.95 (1H,d,J=4.6 Hz), 5.24 (1H,d,J=5.4 Hz), 5.32 (1H,dd,J=4.9, 5.1 Hz), 7.06 (2H,dd,J=7.6, 7.8 Hz), 7.09 (1H,d,J=8.0 Hz), 7.20 (1H,d,J=7.8 Hz), 7.40 (1H,d,J=7.8 Hz), 8.36 (1H,d,J=7.6 Hz), 8.51 (1H,d,J=7.6 Hz), 10.13 (1H,s), 10.52 (1H,s), 11.11 (1H,S)

Example B

The compound represented by the formula ##STR17##

50 mg of rebeccamycin was dissolved in 5 ml of N,N-dimethylformamide, 5 ml of 2N aqueous sodium hydroxide solution was added, and the mixture was stirred 80° C. for 3 hours. 60 ml of water was added to the reaction solution, the mixture was cooled with ice, and the precipitated yellow precipitate was recovered by rich. This was subjected to column chromatography on silica gel (inner diameter 1.5 cm, length 45 cm), the column was washed with chloroform, elution was carried out with chloroform-tetrahydrofuran (10:1), and the fraction containing the desired product was concentrated to dryness. The resultant yellow powder was washed with chloroform to give 6.4 mg of the captioned compound.

Rf value: 0.51 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol: tetrahydrofuran: acetic acid=10:1:1:0.2)

FAB-MS(m/z): 571 [M+H]⁺

¹ H-NMR (300 MHz, DMSO-d₆), δ(ppm): 10.9 (1H,s), 9.0 (1H,d,J=7.8 Hz), 8.92 (1H,d,J=7.8 Hz), 7.78 (2H,t,J=7.8 Hz), 7.53 (1H,d,J=7.8 Hz), 7.50 (1H,d,J=7.8,Hz), 7.03 (1H,d,J=8.9 Hz), 3.96 (2H,m), 3.87 (1H,m), 3.61 (3H,s), 3.54-3.73 (3H,m),

Example C

A process for preparation of 12,13-dihydro-1,11-dihydroxy 13-(β-D-glucopyranosyl)-5H-indolo[2,3-a]pyrrolo[3,4-c]carbazole-5,7(6H)-dione

The Microtetraspora sp. A 34549strain cultured on a slant agar medium was inoculated in 110 ml of a medium (pH 7.2 before sterilization) containing 0.2% glucose, 2.0% dextrin, 0.5% oatmeal, 0.5% defatted rice bran, 0.2% defatted meat bone meal, 0.1% dry yeast, 0.05% magnesium sulfate heptahydrate, 0.05% sodium bormide, 0.5% sodium chloride and 0.1% dipotassium hydrogen phosphate in a 500-ml culturing Erlenmeyer flask, and cultured at 28° C. for 8 days on a rotary shaker (180 rpm). 2 ml of the culture broth was inoculated into 110 ml of a medium having the above composition in each of 20 culturing Erlenmeyer flasks having a capacity of 500 ml, and cultured at 28° C. on a rotary shaker (180 rpm). After culture for 9 days, 0.5 ml of a 20 mg/ml dimethylsulfoxide solution of BE-13793C was added per flask and culture was continued under the same conditions as above for further 15 days.

The resultant culture broths were extracted with 3 L of methyl ethyl ketone (MEK). The resultant MEK extract was concentrated under reduced pressure. The resultant concentrate was extracted with ethyl acetate. The ethyl acetate extract (850 ml) was dehydrated with anhydrous sodium sulfate, and then concentrated to dryness. The residue was subjected to silica gal column chromatography (inner diameter 2 cm, length 30 cm, BW-350 silica gel, produced by Fuji Devision Chemical Co.), and after washing with chloroform--methanol--tetrahydrofuran---28% aqueous ammonia (2:1:3:0.2), eluted with chloroform--methanol--tetrahydrofuran (3:1:1). The fraction containing the desired product was concentrated to dryness, the residue was dissolved in a small quantity of tetrahydrofuran-ethanol (1:3), subjected to sephadex LH-20 column chromatography (inner diameter 1.5 cm, length 87 cm), and eluted with ethanol, and the fraction containing the desired product was concentrated to dryness to give 53.9 mg of the title compound, 12,13-dihydro-1,11-dihydroxy-13-(β-D-glucopyranosyl)-5H-indolo-[2,3-a]pyrrolo[3,4-c]carbazole-5,7(6H)-dione.

Rf value: 0.39 (produced by Merck Co., Kiesel Gel 60 F₂₅₄, developing solvent: chloroform--methanol--tetrahydrofuran--acetic acid=3:1:1:0.1)

HPLC; Rt 8.7 minutes (column: Chromatorex ODS, inner diameter 4.6 mm, length 250 mm, detection: UV 305 nm, flow rate: 1 ml/min, mobile phase: methanol--water =6:4)

HR FAB-MS(m/z): 519.1313

H-NMR (400 MHz, DMSO-D₆), δ(ppm): 11.0 (1H,s), 10.9 (1H,s), 10.3 (1H,brs), 9.93 (1H,brs), 8.69 (1H,d,J=7.8 Hz), 8.51 (1H,d,J=7.8 Hz), 7.17 (2H,t,J=7.8 Hz), 7.05 (1H,d,J=9.3 Hz), 7.01 (1H,d,J=7.8 Hz), 6.99 (1H,d,J=7.8 Hz), 5.4 1 (1H,d,J=5.9 Hz), 5.34 (1H,brs), 5.20 (1H,d,J=5.4 Hz), 4.89 (1H,brs), 4.02 (2H,m), 3.74 (1H,m), 3.63 (2H,m), 3.41 (1H,m)

Example D

A process for preparation of 12,13-dihydro-1,11-dihydroxy-13-(β-D-glucopyranosyl)-5H-indolo[2,3-a]pyrrolo[3,4-c]carbazole-5,7(6H)-dione

The Saccharothrix aerocolonigenes ATCC 39243 strain cultured on a slant agar medium was inoculated in 110 ml of a medium (pH 7.2 before sterilization) containing 3.0% glucose, 1.0% soy flour, 1.0% pharma media and 0.3% calcium carbonate in each of even 500-ml culturing Erlenmeyer flasks, and cultured at 28° C. for 48 hours on a rotary shaker (180 rpm). 4 ml of the culture broth was inoculated into 110 ml of a medium (pH 7.2 before sterilization) containing 1.0% glucose, 6.0% dextrin, 1.5% linseed meal, 0.5% autolyzed yeast, 0.1% ferrous sulfate heptahydrate, 0.1% ammonium dihydrogen phosphate, 0.1% ammonium sulfate and 1.0% calcium carbonate in each of 150 culturing Erlenmeyer flasks having a capacity of 500 ml, and cultured at 28° C. on a rotary shaker (180 rpm). After culture for 120 hours, 0.5 ml of a 22 mg/ml dimethylsulfoxide solution of BE-13793C was added per flask and culture was continued under the same conditions as above for further 120 hours.

The mycelium obtained by filtrating the above culture broth were extracted with methanol (5.1 L and 5.6 L) and tetrahydrofuran (2.2 L and 2.3 L). The methanol and tetrahydrofuran extracts were combined and concentrated to about 1600 ml.

The aqueous concentrate was extracted with hexane (780 ml) to remove impurities and then extracted with ethyl acetate (3 L). The ethyl acetate extract was concentrated to dryness.

The residue was washed with about 90 ml of ethyl acetate and extracted with about 90 ml of methanol. The methanol extract was concentrated to dryness to give 694 mg of yellowish orange solid.

This solid was dissolved in 40 ml of methanol, subjected to sephadex LH-20 column chromatography (3.0×53 cm, produced by Pharmacia) and eluted with methanol. The fractions containing the desired product were combined and concentrated to dryness.

The residue was subjected to silica gel column chromatography (1.5×46 cm, Kiesel Gel 60, produced by Merck Co.), washed successively with chloroform and chloroform-methanol (10:1) and eluted with ethyl acetate-methanol (10:1). The eluent was concentrated to dryness to give 169 mg of the titled compound, 12,13-dihydro-1,11-dihydroxy-13-(β-D-glucopyranosyl)-5H-indolo[2,3-a]pyrrolo[3,4-c]carbazole-5,7(6H)-dione.

The physico-chemical properties of the obtained compound were identical with those of the compound obtained in Example C.

Example 1

The compound represented by the formula ##STR18##

3.51 g of 12,13-dihydro-1,11-dihydroxy-13-(β-D-glucopyranosyl)-5H-indolo[2,3-a]pyrrolo[3,4-c]carbazole-5,7(6H)-dione was dissolved in 8 ml of hydrazine hydrate (Wako Pure Chemical Industries, Ltd.), and reaction was carried out at room temperature for 2 hours. After the reaction, 180 ml of purified water was added, the pH of the solution was adjusted to 5.0 with concentrated hydrochloric acid, the mixture was sufficiently cooled with ice, and the resulting precipitate was collected by filtration, washed with purified water and dried under reduced pressure to give 3.51 g of the captioned compound represented by the formula (1). (yield: 97%)

FAB-MS (m/z): 535 (M+H)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 10.9 (1H, brs), 10.4 (1H, s), 10.0 (1H, s), 8.72 (1H, d, J=7.8 Hz), 8.54 (1H, d, J=7.8 Hz), 7.19 , (2H, t, J=7.8 Hz), 7.19 (2H, t, J=7.8 Hz), 7.05 (1H, d, J=9.3 Hz), 7.02 (1H, d, J=7.8 Hz), 7.00 (1H, d, J=7.8 Hz), 5.42 (1H, brd, J=5. 8 Hz), 5.35 (1H, brs), 5.22 (1H, brd, J=4.4 Hz), 4.96 (2H, brs), 4.91 (1H, brd, J=5.3 Hz), 4.01 (2H, m), 3.73 (1H, m), 3.63 (2H, m), 3.39 (1H, m)

Example 2

The compound represented by the formula ##STR19##

3.47 g of the compound obtained in Example 1 was dissolved in 20 ml of N,N-dimethylformamide (DMF), while the solution was stirred at room temperature, 20 ml of a 100 mg/ml solution of glyoxylic acid (Sigma Co.) was added portionwise, and thereby a precipitate was formed and solidified into a gel-like state. Further, 200 ml of purified water was added, the reaction solution was cooled with ice, and the resultant precipitate was collected by filtration, washed with purified water and dried under reduced pressure to give 3.85 g of the captioned compound represented by the formula (2). (yield: 100)

FAB-MS (m/z): 591 (M+H)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 11.1 (1H, brs), 10.5 (1H, brs), 10.1 (1H, brs), 9.01 (1H, s), 8.69 (1H, d, J=7.8 Hz), 8.53 (1H, d, J=7.8 Hz), 7.23 (2H, t, J=7.8 Hz), 7.10 (1H, d, J=9.3 Hz), 7.06 (1H, d, J=7.8 Hz), 7. 04 (1H, d, J=7.8 Hz), 5.44 (1H, brs), 5.34 (1H, brs), 5.24 (1H, brs), 4.95 (1H, brd, J=5.9 Hz), 4.02 (2H, m), 3.76 (1H, m), 3.64 (2H, m), 3.40 (1H, m)

Example 3

The Compound represented by the formula ##STR20##

24 mg of the compound obtained in Example 1 was dissolved in 0.5 ml of N,N-dimethylformamide (DMF), while the solution was stirred at room temperature, 0.2 ml of succinic semialdehyde (Aldrich Chemical Co.) was added, and one hour later, 5 ml of purified water was added. After the reaction solution was cooled with ice, and the resultant precipitate was collected by filtration, washed with purified water and dried under reduced pressure to give 25.3 mg of the captioned compound represented by the formula (3). (yield: 91%)

FAB-MS (m/z): 619 (M+H)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 12.1 (1H, brs), 11.0 (1H, brs), 10.4 (1H, brs), 10.0 (1H, brs), 8.69 (1H, brs), 8.68 (1H, d, J=7.8 Hz), 8.51 (1H, d, J=8.3 Hz), 7.19 (2H, t, J=7.8 Hz), 7.07 (1H, d, J=9.3 Hz), 7.04 (1H, d, J=7.8 Hz), 7.01 (1H, d, J=7.8 Hz), 5.43 (1H, brd, J=5.4 Hz), 5.33 (1H, brs), 5.22 (1H, brs), 4.93 (1H, brd, J=4.9 Hz), 4.01 (2H, m), 3.74 (1H, m), 3.63 (2H, m), 3.40 (1H, m)

Example 4

The compound represented by the formula ##STR21##

511 mg of rebeccamycin [the compound described in J. Antibiotics 40, 668-678 (1987)] was dissolved in 3 ml of hydrazine hydrate (Wako Pure Chemical Industries, Ltd.), and the solution was allowed to stand at room temperature for one hour. 200 ml of purified water was added, and the resultant precipitate was collected by filtration, washed with 100 ml of purified water and dried under reduced pressure to give 497 mg of the captioned 6-N-aminorebeccamycin represented by the formula (4). (yield: 95%)

FAB-MS (m/z): 585 (M+H)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 10.64 (1H, brs), 9.24 (1H, d, J=7.8 Hz), 9.07 (1H, d, J=7.8 Hz), 7.70 (2H, t, J=7.8 Hz), 7.45 (1H, d, J=7.8 Hz), 7.42 (1H, d, J=7.8 Hz), 6.93 (1H, d, J=8.8 Hz), 5.42 (1H, d, J=5.8 Hz), 5.33 (1H, t, J=5.4 Hz), 5.03 (3H, brs), 3.97 (2H, m), 3.84 (1H, m), 3.59 (3H, s), 3.50˜3.70 (3H, m)

Example 5

The compound represented by the formula ##STR22## [Process A]

5 g of the compound obtained in Example 1 was dissolved in 60 ml of N,N-dimethylformamide, 1.8 ml of concentrated hydrochloric acid was added, the mixture was heated at 60° C. for 4 hours, 0.8 ml of concentrated hydrochloric acid was further added, and the mixture was warmed at 37° C. for 16 hours. This was mixed with 1 l of ethyl acetate, the mixture was washed successively with 2% sodium bicarbonate aqueous solution and water, and then the ethyl acetate layer was dehydrated with anhydrous sodium sulfate and concentrated to dryness to give 3.3 g of orange powder. This was dissolved in methanol and subjected to column chromatography on Sephadex LH 20 (inner diameter 3 cm, length 54 cm, eluted with methanol), and the fractions containing the desired product were concentrated to dryness to give 2413.6 mg of the captioned compound represented by the formula (5) as orange powder.

[Process B]

25.9 mg of the compound obtained in Example A was dissolved in 0.5 ml of N,N-dimethylformamide, 15.0 mg of formic hydrazide was added, and the mixture was stirred at 70° C. for 2 hours. This was mixed with 70 ml of ethyl acetate, and the mixture was washed with water (20 ml). The ethyl acetate layer was dehydrated with anhydrous sodium sulfate and concentrated to dryness to give 26.9 mg of orange powder. This was dissolved in methanol and subjected to column chromatography on Sephadex LH 20 (inner diameter 1.5 cm, length 48 cm, eluted with methanol), and the fractions containing the desired product were concentrated to dryness to give 16.3 mg of the captioned compound represented by the formula (5) as orange powder.

Rf value: 0.35 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform-methanol-tetrahydrofuran =2:1:1)

FAB-MS (m/z): 562 (M)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 11.0 (1H, brs), 10.8 (1H, s), 10.4 (1H, s), 10.0 (1H, s), 8.64 (1H, d, J=8.3 Hz), 8.47 (1H, d, J=8.3 Hz), 8.44 (1H, s), 7.21 (2H, t, J=7.8 Hz), 7.06 (1H, d, J=9.7 Hz), 7.05 (1H, d, J=7.8 Hz), 7.02 (1H, d, J=7.8 Hz), 5.43 (1H, d, J=5.8 Hz), 5.36 (1H, brs), 5.22 (1H, d, J=5.4 Hz), 4.92 (1H, d, J=5.4 Hz), 4.02 (2H, m), 3.75 (1H, m), 3.62 (2H, m), 3.39 (1H, m)

Example 6

The compound represented by the formula ##STR23##

30 ml of acetic acid and 2 ml of acetic anhydride were added to 510 mg of the compound obtained in Example 1, and the compound was dissolved therein with heating at 90° C. Water was added thereto to make the mixture 300 ml, and the reaction product was adsorbed on a column of Diaion HP 20 (inner diameter 3 cm, length 13.5 cm) and after washing the column with 600 ml of water, eluted with 300 ml of methanol. The methanol eluate was concentrated to dryness, the residue was dissolved in 50 ml of methanol, and the solution was concentrated to about 5 ml. 100 ml of ethyl acetate was added thereto, the mixture was allowed to stand overnight at 4° C., and the resultant orange precipitate was collected by filtration to give 426 mg of the captioned compound represented by the formula (6).

Rf value: 0.43 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform-methanol-tetrahydrofuran=2:1:1)

FAB-MS (m/z): 576 (M)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 11.0 (1H, s), 10.7 (1H, s), 10.4 (1H, brs), 10.05 (1H, s), 8.64 (1H, d, J=7.8 Hz), 8.47 (1H, d, J=7.8 Hz), 7.20 (2H, t, J=7.8 Hz), 7.01-7.06 (3H, m), 5.35-5.45 (2H, m), 5.23 (1H, brs), 4.92 (1H, brs), 4.02 (2H, m), 3.74 (1H, m), 3.58-3.70 (2H, m), 3.40 (1H, m), 2.10 (3H, s)

Example 7

The compound represented by the formula ##STR24##

72.5 mg of the compound obtained in Example 1 was dissolved in a mixture of 8 ml of tetrahydrofuran and 5 ml of methanol, 140 μl of 2N hydrochloric acid and 13.2 μl of 37% formaldehyde aqueous solution were added, and the mixture was stirred at room temperature for 2 hours and concentrated to dryness. This was dissolved in 5 ml of N,N-dimethylformamide, 80 mg of 10% palladium carbon, and the mixture was subjected to reduction under hydrogen gas at room temperature for 2 hours and then filtered on Celite. 80 ml of ethyl acetate was added to the resultant filtrate, the mixture was washed successively with 2% sodium bicarbonate aqueous solution and water, and then the resultant ethyl acetate layer was dehydrated and concentrated to dryness to give 28.8 mg of orange powder. This was dissolved in a small quantity of methanol, and the solution was subjected to column chromatography on Sephadex LH-20 (inner diameter 1.5 cm, length 90 cm, eluted with methanol) to give 17.1 mg of the captioned compound represented by the formula (7) as orange powder.

Rf value: 0.49 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform-methanol-tetrahydrofuran-acetic acid=20:10:10:1)

FAB-MS (m/z): 549 (M+H)⁺

¹ H-NMR (400MHz, DMSO-d₆), δ(ppm): 10.9 (1H, s), 10.4 (1H, s), 9.98 (1H, s), 8.72 (1H, d, J=7.8 Hz), 8.54 (1H, d, J=7.8 Hz), 7.19 (2H, t, J=7.8 Hz), 7.00-7.06 (3H, m), 5.73 (1H, q, J=5.4 Hz), 5.43 (1H, d, J=5.7 Hz), 5.35 (1H, brs). 5.22 (1H, d, J=5.4 Hz), 4.90 (1H, d, J=5.4 Hz), 3.96-4.03 (2H, m), 3.74 (1H, m), 3.58-3.70 (2H, m), 3.40 (1H, m), 2.74 (3H, d, J=5.4 Hz)

Example 8

The compound represented by the formula ##STR25##

500 mg of the compound obtained in Example 2 was dissolved in 6 ml of N,N-dimethylformamide(DMF), 75 mg of 10% palladium-carbon (Pd-C) was added, and hydrogenation was carried out at room temperature for 3.5 hours under the stirring. The reaction mixture was filtered using filter paper on which celite was spread to remove Pd-C, and 150 ml of water was added to the filtrate. The mixture was adjusted to pH 5 with 1N NaOH, and then extracted with ethyl acetate (200 ml×5). The ethyl acetate layer was concentrated, and the precipitated crystals were collected by filtration to give 182.3 mg of the captioned compound represented by the formula (8).

FAB-MS (m/z): 593 (M+H)⁺

1H-NMR (400 MHz, DMSO-d₆), δ(ppm): 12.6 (1H, brs), 10.9 (1H, s). 10.4 (1H, s), 10.0 (1H, s), 8.69 (1H, d, J=8.3 Hz), 8.52 (1H, d, J=7.8 Hz), 7.18 (2H, t, J=7.8 Hz), 6.99˜7.05 (3H, m), 5.90 (1H, brs), 5.42 (1H, d, J=5.4 Hz), 5.35 (1H, t, J=5.4 Hz), 5.21 (1H, d, J=4.9 Hz), 4.89 (1H, d, J=5.4 Hz), 4.03 (2H, m), 3.83 (2H, s), 3.74 (1H, m). 3.63 (2H, m), 3.39 (1H, m)

Example 9

The compound represented by the formula ##STR26##

30 ml of methanol was added to 501.7 mg of the compound obtained in Example A and 501.7 mg of semicarbazide hydrochloride, 0.325 ml of triethylamine was then added, and the mixture was refluxed with heating for 8 hours. After the reaction, the reaction solution was concentrated to dryness, 300 ml of methyl ethyl ketone (MEK) and 200 ml of water were added, extraction operation was carried out, and another 300 ml of MEK was added to the water layer to carry out reextration. The MEK layers were combined and concentrated to dryness, 300 ml of methanol was added to the residue to dissolve it, the solution was subjected to a chromatograph tower of Sephadex LH-20 (3×28 cm), and elution was carried out with methanol. The fractions containing the desired product were concentrated to dryness to give 461 mg of the captioned compound represented by the formula (g) as red crystalline powder.

Rf value: 0.15 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol: tetrahydrofuran =2:1:1)

FAB-MS (m/z): 577 (M)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 11.0 (1H, s), 10.4 (1H, s), 10.0 (1H, s). 8.68 (1H, d, J=7.8 Hz), 8.66 (1H, brs), 8.51 (1H, d, J=7.8 Hz), 7.20 (2H, t, J=7.8 Hz), 7.01˜7.07 (3H, m), 6.41 (2H, brs), 5.44 (1H, d, J=5.4 Hz), 5.38 (1H, brs), 5.23 (1H, d, J=4.9 Hz 4.91 (1H, brs), 4.00˜4.09 (2H, m), 3.75 (1H, m), 3.60˜3.68 (2H, m), 3.39 (1H, m)

Example 10

The compound represented by the formula ##STR27##

4 ml of methanol was added to 22 mg of the compound obtained in Example A and 20 mg of thiosemicarbazide, and the mixture was refluxed with heating for 22 hours. The reaction solution was concentrated to dryness, the residue was dissolved in 4 ml of methanol, the solution was subjected to a chromatograph tower of Sephadex LH-20 (1.8×35 cm), and elution was carried out with methanol. The fractions containing the desired product were concentrated to dryness to give 10.7 mg of the captioned compound represented by the formula (10).

Rf value: 0.29 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol:tetrahydrofuran=2:1:1)

FAB-MS (m/z): 594 (M+H)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 11.0 (1H, s), 10.4 (1H, brs), 10.1 (1H, brs), 9.73 (1H, brs). 8.65 (1H, d, J=7.8 Hz), 8.49 (1H, d, J=7.8 Hz), 8.27 (2H, s), 7.21 (2H, t, J=7.8 Hz), 7.01˜7.12 (3H, m), 5.45 (1H, brs), 5.37 (1H, brs), 5.24 (1H, brs), 4.91 (1H, brs), 3.97˜4.10 (2H, m), 3.74 (1H, m), 3.62 (2H, m), 3.40 (1H, m)

Example 11

The compound represented by the formula ##STR28##

9.5 mg of the compound obtained in Example 1 was dissolved in 2 ml of tetrahydrofuran (THF), to the solution was added 30 mg of methanesulfonic anhydride (Aldrich Chemical Co.), and the mixture was allowed to stand at room temperature for 48 hours. The reaction solution was concentrated to dryness, and the residue was dissolved in 2 ml of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.8×34 cm), and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 8.3 mg of the captioned compound represented by the formula (11).

Rf value: 0.48 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol:tetrahydrofuran=2:1:1)

FAB-MS (m/z): 612 (M)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 11.0 (1H, s), 10.5 (1H, brs), 10.4 (1H, s), 10.1 (1H, s). 8.67 (1H, d, J=7.9 Hz), 8.50 (1H, d, J=7.7 Hz), 7.22 (2H, t, J=7.6 Hz), 7.02˜7.07 (3H, m), 5.43 (1H, d, J=5.8 Hz), 5.36 (1H, brs), 5.22 (1H, d, J=5.2 Hz), 4.89 (1H, d, J=4.8 Hz), 4.03 (2H, m), 3.75 (1H, m), 3.63 (2H, m), 3.40 (1H, m)

Example 12

The compound represented by the formula ##STR29##

1 ml of the methanol and 2 ml of tetrahydrofuran were added to 11.7 mg of the compound obtained in Example 1 to make a solution, 0. 1 ml of propionic anhydride (Aldrich Chemical Co.) was added, and the mixture was stirred at room temperature for 4 hours. 2 ml of water and 3 ml of methanol were added to the reaction solution, the mixture was allowed to stand for 30 minutes and then concentrated to dryness, and 3 ml of methanol was added to make a solution. The solution was subjected to a chromatograph tower of Sephadex LH-20 (1.8×30 cm) and eluted with methanol, and the fractions containing the desired product were concentrated to dryness to give 6.2 mg of the captioned compound represented by the formula (12 ).

Rf value: 0.55 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol:tetrahydrofuran=2:1:1)

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 11.0 (1H, s). 10.6 (1H, brs), 10.4 (1H, brs), 10.0 (1H, s), 8.64 (1H, d, J=7.8 Hz), 8.47 (1H, d, J=7.8 Hz), 7.20 (2H, t, J=7.8 Hz), 7.00˜7.08 (3H, m), 5.30˜5.45 (2H, m), 5.21 (1H, m), 4.92 (1H, m), 4.02 (2H, m), 3.75 (1H, m), 3.62 (2H, m), 3.38 (1H, m), 2.39 (2H, q, J=9.3 Hz), 1.16 (3H, t, J=7.3 Hz)

Example 13

The compound represented by the formula ##STR30##

9.9 mg of the compound obtained in Example 1 was dissolved in 2 ml of tetrahydrofuran, 0.06 ml of trifluoroacetic anhydride (Aldrich Chemical Co.) was added, and the mixture was allowed to stand at room temperature for 15 minutes. 2 ml of water was added to the reaction solution, the mixture was concentrated to dryness, 2 ml of water and 10 ml of ethyl acetate were added, extraction operation was carried out, and the resultant ethyl acetate layer was concentrated to dryness. The resultant crude substance was dissolved in 3 ml of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.8×30 cm) and eluted with methanol, and the fractions containing the desired product were concentrated to dryness to give 9.5 mg of the captioned compound represented by the formula (13).

Rf value: 0.53 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol:tetrahydrofuran=2:1:1)

FAB-MS (m/z): 630 (M)⁺

¹ H-NMR (500 MHz, DMSO-d₆), δ(ppm): 12.7 (1H, brs), 11.0 (1H, brs), 10.5 (1H, brs), 10.1 (1H, brs), 8.61 (1H, d, J=7.6 Hz), 8.45 (1H, d, J=7.9 Hz), 7.21 (2H, t, J=7.6 Hz), 7.02˜7.07 (3H, m), 5.42 (1H, d, J=5.8 Hz), 5.35 (1H, brs), 5.21 (1H, brs), 4.91 (1H, d, J=5.5 Hz), 4.02 (2H, m), 3.76 (1H, m), 3.61 (2H, m), 3.39 (1H, m)

Example 14

The compound represented by the formula ##STR31##

4 ml of methanol and 4 ml of benzene were added to 31.6 mg of the compound obtained in Example 8 to make a solution, 0.15 ml of trimethylsilyldiazomethane (10% hexane solution, Tokyo Kasei Co.) was added, and the mixture was allowed to stand at room for 10 minutes and concentrated to dryness to give 29.3 mg of the methyl ester of the compound obtained in Example 8. This was dissolved in 5 ml of methanol, 0.6 ml of concentrated ammonia water was added, and the mixture was stirred at room temperature for 16 hours. The reaction solution was concentrated to dryness, 3 ml of methanol was added to the residue to make a solution, and the solution was subjected to a chromatograph tower of Sephadex LH-20 (1.8×36 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 16.9 mg of the captioned compound represented by the formula (14).

Rf value: 0.22 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol:tetrahydrofuran=2:1:1)

FAB-MS (m/z): 592 (M+H)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 10.9 (1H, s), 10.4 (1H, brs), 10.0 (1H, brs), 8.69 (1H, d, J=7.3 Hz), 8.52 (1H, d, J=8.3 Hz), 7.77 (1H, brs), 7.39 (1H, brs), 7.19 (2H, t, J=7.8 Hz), 6.98˜7.05 (3H, m), 6.25 (1H, t, J=3.9 Hz), 5.41 (1H, d, J=5.4 Hz), 5.35 (1H, brs), 5.20 (1H, d, J=5.4 Hz), 4.87 (1H, d, J=5.4 Hz), 4.02 (2H, m), 3.74 (1H, m), 3.68˜3.70 (4H, m), 3.39 (1H, m)

Example 15 ##STR32##

2 ml of methanol was added to 11 mg of the compound obtained in Example A and 10 mg of 2-hydrazinopyridine (Aldrich Chemical Co.) to make a solution, and the solution was refluxed with heating for 1.5 hours. The reaction solution was concentrated to dryness, 30 ml of water and 50 ml of ethyl acetate were added, the water layer was adjusted to pH 5 with 1N hydrochloric acid, extraction operation was carried out, and the resultant ethyl acetate layer was concentrated to dryness. The resultant crude substance was dissolved in 2 ml of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.8×36 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 10 mg of the captioned compound represented by the formula (15).

Rf value: 0.46 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol:tetrahydrofuran=2:1:1)

FAB-MS (m/z): 612 (M+H)⁺

¹ H-NMR (400MHz, DMSO-d₆), δ(ppm): 11.0 (1H, s), 10.4 (1H, s), 10.0 (1H, s), 9.34 (1H, s), 8.65 (1H, d, J=8.3 Hz), 8.48 (1H, d, J=7.8 Hz), 7.95 (1H, d, J=4.9 Hz), 7.62 (1H, t, J=7.8 Hz), 7.18 (2H, t, J=7.8 Hz), 7.00˜7.08 (3H, m), 6.86 (1H, d, J=7.8 Hz), 6.78 (1H, dd, J=4.9, 7.8 Hz), 5.44 (1H, d, J=5.8 Hz), 5.37 (1H, brs), 5.23 (1H, d, J=5.8 Hz), 4.92 (1H, brs), 4.02 (2H, m), 3.76 (1H, m). 3.64 (2H, m), 3.41 (1H, m)

Example 16

The compound represented by the formula ##STR33##

4 ml of methanol was added to 24 mg of the compound obtained in Example A and 4-hydrazinobenzoic acid (Aldrich Chemical Co.), and the mixture was refluxed with heating for 2 hours. The reaction solution was subjected to a chromatograph tower of Sephadex LH-20 (1.8×44 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 20.9 mg of the captioned compound represented by the formula (16) as red crystalline powder.

Rf value: 0.31 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol:tetrahydrofuran=2:1:1)

FAB-MS (m/z): 655 (M+H)⁺

¹ H-NMR (500 MHz, DMSO-d₆), δ(ppm): 11.0 (1H, s), 10.5 (1H, brs), 10.1 (1H, brs), 9.11 (1H, s), 8.65 (l1H, d, J=7.9 Hz), 8.48 (1H, d, J=7.9 Hz), 7.80 (2H, d, J=8.3 Hz), 7.18 (2H, t, J=7.6 Hz), 7.01˜7.08 (3H, m), 6.84 (2H, d, J=8.3 Hz), 5.20˜5.60 (3H, brs), 4.96 (1H, brs), 4.03 (2H, m), 3.76 (1H, m), 3.65 (2H, m), 3.41 (1H, m)

Example 17

The compound represented by the formula ##STR34##

6 ml of 50% methanol was added to 26 mg of the compound obtained in Example A and 38 mg of oxamic hydrazine (Aldrich Chemical Co.), and the mixture was stirred with heating at 80° C. for 20 hours. The reaction solution was concentrated to dryness, 15 ml of water and 50 ml of ethyl acetate were added, the mixture was adjusted to pH with 1N hydrochloric acid, and extraction operation was carried out. The ethyl acetate layer was concentrated, and the precipitated crystals were collected by filtration to give 10 mg of the captioned compound represented by the formula (17).

Rf value: 0.38 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol:tetrahydrofuran=2:1:1)

FAB-MS (m/z): 606 (M+H)⁺

¹ H-NMR (500 MHz, DMSO-d₆), δ(ppm): 11.4 (1H, s), 11.0 (1H, s), 10.4 (1H, s), 10.0 (1H, s), 8.63 (1H, d, J=7.9 Hz), 8.46 (1H, d, J=7.9 Hz), 8.38 (1H, s), 8.11 (1H, s), 7.21 (2H, t, J=7.9 Hz), 7.02˜7.07 (3H, m), 5.41 (1H, d, J=5.8 Hz), 5.35 (1H, t, J=5.8 Hz), 5.19 (1H, d, J=5.2 Hz), 4.89 (1H, d, J=5.5 Hz), 4.03 (2H, m), 3.76 (1H, m), 3.63 (2H, m), 3.40 (1H, m)

Example 18

The compound represented by the formula ##STR35##

26.7 mg of the compound obtained in Example 1 and 5.5-5 mg of succinic anhydride were dissolved in 0.5 ml of pyridine, and the solution was stirred at room temperature for 18 hours. This was concentrated to dryness under reduced pressure and the residue was dissolved in a small quantity of N,N-dimethylformamide and subjected to high performance liquid chromatography (HPLC) [Chromatolex ODS, 20×250 mm, moving phase: 20% acetonitrile]. The fractions containing the desired product were concentrated to remove acetonitrile, adjusted to pH 2 and extracted with 100 ml of ethyl acetate. The ethyl acetate layer was dehydrated with anhydrous sodium sulfate and concentrated to dryness. The residue was dissolved in methanol and subjected to column chromatography on Sephadex LH-20 (inner diameter 1.5 cm, length 90 cm, eluted with methanol), and the fractions containing the desired product were concentrated to dryness to give 9.7 mg of the captioned compound represented by the formula (18) as orange powder.

HPLC; Rt, 5.3 minutes (column: Chromatolex ODS, inner diameter 4.6 mm, length 250 mm, detection; UV 305 nm, flow rate; 1 ml/minute, moving phase; 27.5% acetonitrile: trifluoroacetic acid=1000:1)

FAB-MS (m/z): 657 (M+Na)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 11.0 (1H, s), 10.7 (1H, brs), 10.4 (1H, brs), 10.1 (1H, brs), 8.64 (1H, d, J=7.9 Hz), 8.47 (1H, d, J=7.9 Hz), 7.19 (2H, t, J=7.8 Hz), 7.01˜7.07 (3H, m), 5.42 (2H, brs), 5.22 (1H, brs), 4.92 (1H, brs). 4.02 (2H, m), 3.75 (1H, m), 3.63 (2H, m), 3.40 (1H, m), 2.65 (2H, t, J=7.3 Hz). 2.52 (2H, t, J=7.3 Hz)

Example 19

The compound represented by the formula ##STR36##

30 mg of the compound obtained in Example 1 was dissolved in 0.5 ml of N,N-dimethylformamide, 0.1 ml of methyl iodide was added, and the mixture was stirred at room temperature for 18 hours. This was mixed with 50 ml of ethyl acetate, the mixture was washed successively with 1% sodium bicarbonate aqueous solution and then water, and the ethyl acetate layer was dehydrated with anhydrous sodium sulfate and concentrated to dryness. The residue was dissolved in methanol and subjected to column chromatography on Sephadex LH-20 (1.5×90 cm, eluted with methanol), and the fractions containing the desired product were concentrated to dryness to give 18.0 mg of the captioned compound represented by the formula (19) as orange powder.

Rf value: 0.51 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol:tetrahydrofuran:acetic acid=20:10:10:1)

FAB-MS (m/z): 563 (M+H)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 10.9 (1H, s), 10.3 (1H, s), 9.95 (1H, s), 8.70 (1H, d, J=8.3 Hz), 8.53 (1H, d, J=8.3 Hz), 7.18 (2H, t, J=7.8 Hz), 7.00˜7.06 (3H, m), 5.41 (1H, d, J=5.4 Hz), 5.34 (1H, t, J=5.4 Hz), 5.19 (1H, d, J=5.4 Hz). 4.86 (1H, d, J=5.4 Hz), 4.02 (2H, m), 3.75 (1H, m), 3.62 (2H, m), 3.39 (1H, m), 3.0 2 (6H, s)

Example 20

The compound represented by the formula ##STR37##

82.1 mg of t-butyloxycarbonyl (Boc)-glycine was dissolved in 1 ml of methylene chloride, the solution was stirred under ice cooling for 15 minutes, 96.7 mg of dicyclohexylcarbodiimide dissolved in 1 ml of methylene chloride was added, and the mixture was stirred under ice cooling for 15 minutes. To this was added 227.6 mg of the compound obtained in Example 1 dissolved in 6 ml of pyridine, and the mixture was stirred at room temperature for 17 hours. The reaction solution was concentrated to dryness, the residue was dissolved in ethyl acetate, the solution was washed successively with saturated saline, acidic water (pH 2) and then water, and the ethyl acetate layer was dehydrated with anhydrous sodium sulfate and concentrated to dryness. The residue was subjected to silica gel column chromatography (1.5×55 cm, eluted with toluene:methanol=6:1), and the fractions containing the desired product were concentrated to dryness to give 105.2 mg of the Boc derivative of the captioned compound represented by the formula (20) as orange powder. This was dissolved in 1.2 ml of trifluoroacetic acid, and the solution was stirred at room temperature for 30 minutes to remove the Boc group. The reaction solution was concentrated to dryness, the residue was dissolved in 15 ml of water, and the solution was adjusted to pH 7.5-8 and extracted with n-butanol. 40 ml of water was added to the n-butanol layer (50 ml), and the mixture was adjusted to pH 2 with dilute hydrochloric acid and concentrated to dryness. The resultant orange powder was dissolved in methanol and subjected to column chromatography on Sephadex LH-20 (1.5×38 cm, eluted with methanol), and the fractions containing the desired product were concentrated to dryness to give 63.7 mg of the hydrochloride of the captioned compound represented by the formula (20) as orange powder.

HPLC; Rt, 8.7 minutes (column:Chromatolex ODS, inner diameter 4.6 mm, length 250 mm, detection; UV 305 nm, flow rate; 1 ml/minute, moving phase; 20% acetonitrile: trifluoroacetic acid=1000:1→70% acetonitrile:trifluoroacetic acid=1000.1, 30 minutes linear gradient)

FAB-MS(m/z): 592 (M+H)⁺

¹ H-NMR (hydrochloride, 400 MHz, DMSO-d₆), δ(ppm): 11.3 (1H, brs), 11.0 (1H, brs), 10.5 (1H, s), 10.1 (1H, s). 8.62 (1H, d, J=8.3 Hz), 8.46 (1H, d, J=8.3 Hz), 8.31 (2H, s). 7.19 (2H, t, J=7.8 Hz), 7.0˜7.08 (3H, m), 5.46 (1H, brs), 5.34 (1H, brs), 5.27 (1H, brs), 4.91 (1H, brd, J=4.9 Hz), 4.03 (2H, m), 3.98 (2H, s), 3.76 (1H, m), 3.64 (2H, m), 3.40 (1H, m)

Example 21

The compound represented by the formula ##STR38##

40.0 mg of the compound obtained in Example A was dissolved in 3 ml of N,N-dimethylformamide, 42.2 mg of 1-deoxy-1-hydrazino-D-sorbitol and 0.1 ml of triethylamine was added, and the mixture was refluxed with heating for 16 hours. This was brought back to room temperature, subjected to a chromatograph tower of Sephadex LH-20 (1.8×20 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 20.0 mg of the captioned compound represented by the formula (21).

FAB-MS (m/z): 699 (M+H)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 10.91 (1H, s), 10.35 (1H, brs), 9.96 (1H, brs), 8.73 (1H, d, J=8.9 Hz), 8.54 (1H, d, J=8.9 Hz), 7.20 (2H, t, J=8.4 Hz), 7.00-7.10 (3H, m), 5.76 (1H, t, J=3.8 Hz), 5.42 (1H, d, J=5.5 Hz), 5.37 (1H, brs), 5.22 (1H, d, J=5.5 Hz), 4.89 (1H, brs), 4.67 (1H, d, J=3.4 Hz), 4.45 (1H, d, J=5.1 Hz), 4.37 (1H, d, J=7.0 Hz), 4.25-4.43 (2H, m), 4.00 (2H, m), 3.55-3.80 (7H, m), 3.44-3.52 (2H, m), 3.35-3.44 (2H, m), 3.05-3.20 (2H, m)

Example 22

The compound represented by the formula ##STR39##

100 mg of the compound obtained in Example A was dissolved in 5 ml of N,N-dimethylformamide, 100 mg of carbohydrazide was added, and the mixture was stirred at 80° C. for 3 hours and concentrated to dryness. The residue was dissolved in methanol and the insoluble matters were removed by Celite filtration. The resultant filtrate was concentrated, and the residue was dissolved in a small quantity of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.5×20 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 91.2 mg of the captioned compound represented by the formula (22).

Rf value: 0.1 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform-methanol-tetrahydrofuran=2:1:1:)

FAB-MS (m/z): 593 (M+H)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 10.96 (1H, s), 10.40 (1H, s). 10.01 (1H, s), 8.95 (1H, s), 8.65 (1H, d, J=8.2 Hz), 8.50 (1H, d, J=8.2 Hz), 7.90 (1H, s), 7.17 (2H, t, J=6.9 Hz), 7.00-7.10 (3H, m), 5.43 (1H, d, J=4.1 Hz), 5.38 (1H, brs), 5.20 (1H, s), 4.90 (1H, s), 4.39 (2H, brs), 4.04 (2H, m), 3.75 (1H, m), 3.55-3.70 (2H, m), 3.38 (1H, m)

Example 23

The compound represented by the formula ##STR40##

15.0 mg of the compound obtained in Example A was dissolved in 1 ml of N,N-dimethylformamide, 32 mg of 3-hydoxybenzylhydrazine dihydrochloride and 0.1 ml of 10% sodium bicarbonate aqueous solution were added, and the mixture was stirred at 80° C. for 4 hours. This was mixed with 50 ml of ethyl acetate, the mixture was washed successively with 0.2N hydrochloric acid and then saturated saline, and the ethyl acetate layer was dehydrated with anhydrous sodium sulfate and concentrated to dryness. The residue was dissolved in a small quantity of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.8×15 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 15.3 mg of the captioned compound represented by the formula (23).

Rf value: 0.22 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform-methanol-tetrahydrofuran=5:1:1)

FAB-MS (m/z): 641 (M+H)⁺

¹ H-NMR (200 MHz, DMSO-d₆), δ(ppm): 10.90 (1H, s), 10.38 (1H, s), 9.99 (1H, s), 9.30 (1H, s), 8.70 (1H, d, J=8.1 Hz), 8.53 (1H, d, J=8.5 Hz), 6.86-7.22 (8H, m), 6.61 (1H, dd, J=2.2, 8.4 Hz), 6.03 (1H, t, J=5.1 Hz), 5.43 (1H, d, J=5.4 Hz), 5.35 (1H, t, J=5.0 Hz), 5.22 (1H, d, J=5.4 Hz), 4.89 (1H, d, J=5.4 Hz), 4.19 (2H, d, J=5.1 Hz), 4.00 (2H, m), 3.72 (1H, m), 3.53-3.70 (2H, m), 3.38 (1H, m)

Example 24

The compound represented by the formula ##STR41##

64.6 mg of the compound obtained in Example A was dissolved in 2 ml of N,N-dimethylformamide, 30 mg of 2-cyanoethylhydrazine, and the mixture was stirred at ° C for 1.5 hours. 50 ml of 0.2N hydrochloric acid was added to the reaction solution, and the mixture was extracted with ethyl acetate (50 ml×2). The ethyl acetate layer was concentrated to dryness, and the residue was dissolved in a small quantity of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.8×30 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 45.0 mg of the captioned compound represented by the formula (24).

Rf value: 0.39 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol=3:1)

FAB-MS (m/z): 588 (M+H)⁺

¹ H-NMR (200 MHz, DMSO-d₆), δ(ppm): 10.91 (1H, s), 10.36 (1H, s), 9.98 (1H, s), 8.70 (1H, d, J=8.4 Hz), 8.53 (1H, d, J=8.4 Hz), 7.18 (2H, t, J=8.4 Hz), 6.95-7.10 (3H, m), 6.15 (1H, t, J=4.2 Hz), 5.42 (1H, d, J=5.7 Hz), 5.34 (1H, brs), 5.23 (1H, d, J=4.4 Hz), 4.91 (1H, d, J=5.3 Hz), 4.00 (2H, m), 3.72 (1H, m), 3.55-3.70 (2H, m), 3.39 (1H, m), 3.30 (2H, t d, J=4.2, 6.2 Hz), 2.69 (2H, t, J=6.2 Hz)

Example 25

The compound represented by the formula ##STR42##

1.09 g of the compound obtained in Example A was dissolved in 35 ml of N,N-dimethylformamide-2 ml of water, 455 mg of 2-hydrazino-2-imidazoline hydrobromide and 211 mg of sodium bicarbonate were added, and the mixture was stirred at 80° C. for 2 hours and concentrated to dryness. The residue was dissolved in 300 ml of 0.2N hydrochloric acid and extracted with n-butanol (1 L×2). The butanol layer was concentrated to dryness, and the residue was dissolved in a small quantity of methanol, subjected to a chromatograph tower of Sephadex LH-20 (3.0×80 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 650 mg of the captioned compound represented by the formula (25).

Rf value: 0.55 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; n-butanol:acetic acid:water=4:1:1)

FAB-MS (m/z): 603 (M+H)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 11.2 (1H, s), 10.90 (1H, brs), 10.50 (1H, s), 10.14 (1H, s), 9.42 (1H, brs), 8.92 (1H, brs), 8.62 (1H, d, J=10.6 Hz). 8.45 (1H, d, J=9.5 Hz), 7.22 (2H, t, J=6.5 Hz), 7.02-7.10 (3H, m), 5.48 (1H, d, J=4.7 Hz), 5.32 (2H, brm), 4.94 (1H, d, J=3.5 Hz), 4.04 (2H, m), 3.70-3.90 (5H, m), 3.54-3.70 (2H, m), 3.41 (1H, m)

Example 26

The compound represented by the formula ##STR43##

48.3 mg of t-he compound obtained in Example A was dissolved in 1 ml of N,N-dimethylformamide, 14.3 mg of 1-amino-4-(2-hydroxyethyl) piperazine and 0.1 ml of saturated sodium bicarbonate aqueous solution were added, and the mixture was stirred at 80° C. for 2 hours. This was distributed between 50 ml of ethyl acetate and 50 ml of water, 5 ml of 0.2N hydrochloric acid was added to the water layer, and the mixture was extracted with n-butanol (100 ml×2). The butanol layer was concentrated to dryness, and the residue was dissolved in a small quantity of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.8×30 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 22 mg of the captioned compound represented by the formula (26).

Rf value: 0.53 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; n-butanol:acetic acid:water=4:1:1)

FAB-MS (m/z): 648 (M+H)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 10.92 (1H, s), 10.50 (2H, brs), 10.10 (1H, s), 8.66 (1H, d, J=7.2 Hz), 8.50 (1H, d, J=8.9 Hz), 7.18 (2H, t, J=8.9 Hz), 7.02-7.12 (3H, m), 5.46 (1H, d, J=5.6 Hz), 5.25-5.40 (3H, brm), 4.86 (1H, d, J=5.6 Hz), 3.95-4.20 (4H, m), 3.70-3.90 (4H, m), 3.55-3.70 (4H, m), 3.20-3.50 (6H, m)

Example 27

The compound represented by the formula ##STR44##

24 mg of the compound obtained in Example A was dissolved in 0.6 ml of N,N-dimethylformamide, 10 mg of t-butyl carbazinate acid was added, and the mixture was stirred at 80° C. for 6 hours. This was mixed with 50 ml of ethyl acetate, the mixture was washed successively with water (30 ml×2) and saturated saline, and the ethyl acetate layer was dehydrated with anhydrous sodium sulfate and concentrated to dryness. The residue was dissolved in 1 ml of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.6×20 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 27.2 mg of the captioned compound represented by the formula (27).

Rf value: 0.42 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol=4:1)

FAB-MS (m/z): 634 (M+H)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 10.99 (1H, s), 10.42 (1H, s), 10.02 (1H, s), 9.82 (1H, brs), 8.65 (1H, d, J=7.7 Hz), 8.49 (1H, d, J=7.7 Hz), 7.18 (2H, t, J=7.7 Hz), 7.00-7.10 (3H, m), 5.42 (1H, brs), 5.35 (1H, brs), 5.21 (1H, brs), 4.90 (1H, brs), 4.02 (2H, m), 3.72 (1H, m). 3.56-3.70 (2H, m), 3.40 (1H, m), 1.50 (9H, s)

Example 28

The compound represented by the formula ##STR45## and the compound represented by the formula ##STR46##

177 mg of the compound obtained in Example 1 was dissolved in 6 ml of N,N-dimethylformamide, 0.68 ml of allyl bromide was added, and the mixture was stirred at room temperature for 1 day. 200 ml of water was added to the mixture, the mixture was extracted with ethyl acetate (200 ml×3), and the ethyl acetate layer was dehydrated with saturated saline and concentrated to dryness. The residue was dissolved in 3 ml of methanol, subjected to a chromatograph tower of Sephadex LH-20 (2.5×40 cm) and eluted with methanol. The fractions containing the desired products respectively were concentrated to dryness to give 42.1 mg of the captioned compound represented by the formula (28) and 67.5 mg of the compound represented by the formula (29).

The compound represented by the formula (28)

Rf value: 0.68 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol=2:1)

FAB-MS (m/z): 575 (M+H)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 10.90 (1H, s), 10.38 (1H, s), 9.98 (1H, s), 8.70 (1H, d, J=9.0 Hz), 8.52 (1H, d, J=10.2 Hz), 7.20 (2H, t, J=7.7 Hz), 6.95-7.08 (3H, m), 5.92 (2H, m), 5.40 (1H, d, J=6.4 Hz), 5.32 (1H, m), 5.20 (2H, m), 5.05 (1H, d, J=11.5 Hz), 4.88 (1H, d, J=5.8 Hz), 4.00 (2H, m), 3.67-3.78 (3H, m), 3.58-3.65 (2H, m), 3.35 (1H, m)

The compound represented by the formula (29)

Rf value: 0.75 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol=2:1)

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 10.91 (1H, s), 10.40 (1H, brs), 10.00 (1H, brs), 8.66 (1H, d, J=9.4 Hz), 8.50 (1H, d, J=9.4 Hz), 7.18 (2H, t, J=8.0 Hz), 7.00-7. 10 (3H, m), 5.90 (2H, ddt, J=6.3, 10.2, 17.0 Hz), 5.42 (1H, d, J=5.3 Hz), 5.33 (1H, brs), 5.23 (2H, d, J=17.0 Hz), 5.22 (1H, brs), 5.04 (2H, d, J=10.2 Hz), 4.91 (1H, brs), 4.02 (2H, m), 3.97 (4H, d, J=6.3 Hz), 3.70 (1H, m). 3.51-3.66 (2H, m), 3.35 (1H, m)

Example 29

The compound represented by the formula ##STR47## and the compound represented by the formula ##STR48##

20 mg of the compound obtained in Example 1 was dissolved in 1 ml of N,N-dimethylformamide, 0.3 ml of benzyl bromide was added, and the mixture was stirred overnight. This was mixed with 40 ml of ethyl acetate, the mixture was washed successively with water (30 ml×2) and and then saturated saline, and the ethyl acetate layer was dehydrated with anhydrous sodium sulfate and concentrated to dryness. The residue was dissolved in 1 ml of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.6×30 cm) and eluted with methanol. The fractions containing the desired products respectively were concentrated to dryness to give 13.2 mg of the captioned compound represented by the formula (30) and 7.2 mg of the compound represented by the formula (31).

The compound represented by the formula (30)

Rf value: 0.44 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol=3:1)

FAB-MS (m/z): 715 (M+H)⁺

¹ H-NMR (200 MHz, DMSO-d₆), δ(ppm): 10.85 (1H, s), 10.35 (1H, s), 9.96 (1H, s), 8.65 (1H, d, J=8.5 Hz), 8.45 (1H, d, J=9.0 Hz), 7.50-7.65 (4H, m), 7.10-7.40 (8H, m), 6.95-7.10 (3H, m), 5.40 (1H, d, J=5.4 Hz), 5.30 (1H, brs), 5.18 (1H, d, J=4.9 Hz), 4.83 (1H, d, J=4.9 Hz), 4.58 (2H, s), 4.55 (2H, s), 4.00 (2H, m), 3.46-3.80 (3H, m), 3.36 (1H, m)

The compound represented by the formula (31)

Rf value: 0.38 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol=3:1)

FAB-MS (m/z): 625 (M+H)⁺

¹ H-NMR (300 MHz, DMSO-d₆), δ(ppm): 10.88 (1H, s), 10.40 (1H, brs), 10.00 (1H, brs), 8.67 (1H, d, J=7.9 Hz), 8.51 (1H, d, J=7.3 Hz), 7.50 (2H, d, J=6.9 Hz), 7.30 (2H, t, J=6.9 Hz), 7.21 (1H, t, J=6.9 Hz), 7.16 (2H, t, J=7.3 Hz), 6.96-7.07 (3H, m), 6.13 (1H, t, J=5.3 Hz), 5.42 (1H, d, J=5.9 Hz), 5.21 (1H, d, J=5.3 Hz), 4.91 (1H, brs), 4.55 (1H, brs), 4.28 (2H, d, J=5.3 Hz), 4.02 (2H, m), 3.72 (1H, m), 3.55-3.70 (2H, m), 3.40 (1H, m)

Example 30

The compound represented by the formula ##STR49##

1.4 g of the compound obtained in Example A was dissolved in 30 ml of N,N-dimethylformamide, 1 g of methyl carbazate was added, and the mixture was stirred at 80° C. for 2 hours. 400 ml of water was added to the mixture, and the mixture was extracted with ethyl acetate (500 ml×3). The resultant ethyl acetate layer was concentrated to dryness. The residue was dissolved in 5 ml of methanol, subjected to a chromatograph tower of Sephadex LH-20 (3.0×80 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 1.3 g of the captioned compound represented by the formula (32).

Rf value: 0.18 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol=4:1)

FAB-MS (m/z): 592 (M+H)⁺

¹ H-NMR (300 MHz, DMSO-d₆), δ(ppm): 11.00 (1H, s), 10.42 (1H, brs), 10.18 (1H, s), 10.04 (1H, brs), 8.64 (1H, d, J=7. 6 Hz), 8.47 (1H, d, J=8.3 Hz), 7.20 (2H, t, J=8.3 Hz), 7.00-7.10 (3H, m), 5.42 (1H, brs), 5.35 (1H, brs), 5.21 (1H, brs), 4.91 (1H, brs), 4.02 (2H, m), 3.75 (3H, s), 3.50-3.70 (3H, m), 3.40 (1H, m)

Example 31

The compound represented by the formula ##STR50##

90 mg of the compound obtained in Example A was dissolved in 1 ml of N,N-dimethylformamide, 67 mg of (2R,3S)-3,4-O-isopropylidene-2,3,4-trihydroxybutane carbohydrazide, and the mixture was stirred at 80° C. for 7 hours and then at room temperature for 3 days. 50 ml of water was added to the mixture, and the mixture was extracted with ethyl acetate (50 ml×2). The resultant ethyl acetate layer was concentrated to dryness. The residue was dissolved in 3 ml of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.8×25 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 112 mg of the captioned compound represented by the formula (33).

Rf value: 0.14 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol=4:1)

FAB-MS (m/z): 692 (M+H)⁺ ¹ H-NMR (300 MHz, DMSO-d₆), δ(ppm): 11.01 (1H, s), 10.70 (1H, s), 10.45 (1H, s), 10.05 (1H, s), 8.75 (1H, d, J=7.4 Hz), 8.47 (1H, d, J=7.4 Hz), 7.21 (2H, t, J=7.4 Hz), 7.00-7.10 (3H, m), 6.26 (1H, d, J=6.7 Hz), 5.44 (1H, d, J=5.9 Hz), 5.39 (1H, brs), 5.24 (1H, d, J=5.9 Hz), 4.93 (1H, d, J=5.9 Hz), 4.31 (1H, dd, J=6.7, 11.9 Hz), 4.22 (1H, t, J=6.7 Hz), 4.10 (1H, ddd, J=6.7, 6.7, 11.9 Hz), 4.05 (2H, m), 3.91 (1H, t, J=6.7 Hz), 3.76 (1H, m), 3.57-3.71 (2H, m), 3.40 (1H, m), 1.45 (3H, s), 1.36 (3H, s)

Example 32

The compound represented by the formula ##STR51##

25 mg of the compound obtained in Example 1 was dissolved in 5 ml of anhydrous tetrahydrofuran, 10 mg of p-toluenesulfonic anhydride was added, and the mixture was stirred at room temperature for 1 day. The reaction solution was concentrated to dryness, and the residue was dissolved in 1 ml of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.8×20 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 12.3 mg of the captioned compound represented by the formula (34).

Rf value: 0.49 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol:tetrahydrofuran=3:1:1)

FAB-MS (m/z): 688 (M+H)⁺

¹ H-NMR (300 MHz, DMSO-d₆), δ(ppm): 10.98 (1H, s), 10.87 (1H, s), 10.42 (1H, s), 10.05 (1H, s), 8.54 (1H, d, J=7.9 Hz), 8.38 (1H, d, J=7.9 Hz), 7.84 (2H, d, J=8.7 Hz), 7.44 (2H, d, J=8.7 Hz), 7.19 (2H, t, J=7.9 Hz), 7.00-7.08 (3H, m), 5.43 (1H, d, J=4.7 Hz), 5.35 (1H, brs), 5.23 (1H, d, J=4.9 Hz), 4.90 (1H, d, J=4.4 Hz), 4.04 (2H, m), 3.75 (1H, m), 3.55-3.70 (2H, m), 3.40 (1H, m), 2.42 (3H, s)

Example 33

The compound represented by the formula ##STR52##

20 mg of the compound obtained in Example 1 was dissolved in 2 ml of tetrahydrofuran, 0.1 ml of phenyl isocyanate was added, and the mixture was stirred at room temperature for 2 hours. The reaction solution was concentrated to dryness, and the residue was dissolved in ml of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.6×30 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 12 mg of the captioned compound represented by the formula (35).

Rf value: 0.38 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol:tetrahydrofuran=2:1:1)

FAB-MS (m/z): 653 (M+H)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 11.00 (1H, s), 10.40 (1H, brs), 10.10 (1H, brs), 9.48 (1H, s), 9.50 (1H, s), 8.67 (1H, d, J=8.3 Hz), 8.50 (1H, d, J=8.3 Hz), 7.48 (2H, d, J=7.8 Hz), 7.27 (2H, t, J=7.8 Hz), 7.20 (2H, t, J=7.8 Hz), 6.95-7.10 (4H, m), 5.43 (1H, d, J=4.2 Hz), 5.30 (1H, brs), 5.23 (1H, brs), 4.95 (1H, brs), 4.03 (2H, m), 3.75 (1H, m), 3.58-3.70 (2H, m), 3.38 (1H, m)

Example 34

The compound represented by the formula ##STR53##

15 mg of the compound obtained in Example 1 was dissolved in 2 ml of tetrahydrofuran, 16 μl of benzoyl chloride was added, and the mixture was stirred at room temperature for 2 hours. The solvent was distilled away, and the residue was dissolved in 1 ml of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.6×20 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 12 mg of the captioned compound represented by the formula (36).

Rf value: 0.57 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol:tetrahydrofuran=2:1:1)

FAB-MS (m/z): 639 (M+H)⁺

¹ H-NMR (200 MHz, DMSO-d₆), δ(ppm): 11.35 (1H, brs), 11.04 (1H, s), 10.45 (1H, brs), 10.08 (1H, brs), 8.66 (1H, d, J=8 Hz), 8.49 (1H, d, J=8.5 Hz), 8.04 (2H, d, J=7.1 Hz), 7.55-7.78 (3H, m), 7.20 (2H, t, J=8.5 Hz), 7.00-7.15 (3H, m), 5.45 (2H, brs), 5.25 (1H, brs), 4.97 (1H, brs), 4.02 (2H, m), 3.55-3.82 (3H, m), 3.41 (1H, m)

Example 35

The compound represented by the formula ##STR54##

25 mg of the compound obtained in Example A was dissolved in 1.5 ml of N,N-dimethylformamide, 30 mg of α-picolinohydrazide was added, and the mixture was stirred at 80° C. for 2 hours. This was mixed with 50 ml of ethyl acetate, and the mixture was washed successively with water and then saturated saline, dehydrated with anhydrous sodium sulfate, and concentrated to dryness. The residue was dissolved in 1 ml of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.8×15 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 30 mg of the captioned compound represented by the formula (37).

Rf value: 0.58 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol:tetrahydrofuran=2:1:1)

FAB-MS (m/z): 640 (M+H)⁺

¹ H-NMR (300 MHz, DMSO-d₆), δ(ppm): 11.43 (1H, s), 11.02 (1H, s), 10.45 (1H, s), 10.07 (1H, s), 8.82 (1H, d, J=4.2 Hz), 8.75 (1H, d, J=7.3 Hz), 8.48 (1H, d, J=7.8 Hz), 8.12 (2H, m), 7.75 (1H, m), 7.20 (2H, t, J=7.0 Hz), 7.00-7.15 (3H, m), 5.45 (1H, d, J=6.3 Hz), 5.40 (1H, brs), 5.25 (1H, d, J=6.3 Hz), 4.96 (1H, brs), 4.04 (2H, m), 3.76 (1H, m), 3.55-3.72 (2H, m), 3.42 (1H, m)

Example 36

The compound represented by the formula ##STR55##

30 mg of the compound obtained in Example A was dissolved in 1 ml of N,N-dimethylformamide, 30 mg of 2-hydrazinoethanol was added, and the mixture was stirred at 80° C. for 2 hours. This was concentrated to dryness. The residue was dissolved in 1 ml of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.8×20 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 32 mg of the captioned compound represented by the formula (38).

Rf value: 0.32 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol=2:1)

¹ H-NMR (300 MHz, DMSO-d₆), δ(ppm): 10.91 (1H, s), 10.35 (1H, brs), 9.98 (1H, brs), 8.70 (1H, d, J=6.7 Hz), 8.53 (1H, d, J=6.9 Hz), 7.18 (2H, t, J=7.6 Hz), 6.99-7.06 (3H, m), 5.76 (1H, t, J=5.2 Hz), 5.41 (1H, d, J=5.6 Hz), 5.32 (1H, brs), 5.20 (1H, d, J=5.2 Hz), 4.90 (1H, brs), 4.51 (1H, t, J=4.9 Hz), 3.96-4.06 (2H, m), 3.73 (1H, m), 3.55-3.70 (4H, m), 3.39 (1H, m), 3.12 (2H, m)

Example 37

The compound represented by the formula ##STR56##

40 mg of the compound obtained in Example A was dissolved in 2 ml of N,N-dimethylformamide, 10 mg of 1-aminopyrrolidine hydrochloride and 0.1 ml of sodium bicarbonate aqueous solution were added, and the mixture was stirred at 80° C. for 2 hours. 40 ml of water was added thereto and the mixture was extracted with ethyl acetate (40 ml×2). The resultant ethyl acetate layer was dehydrated with anhydrous sodium sulfate, and concentrated to dryness. The residue was dissolved in 1 ml of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.8×20 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 10.0 mg of the captioned compound represented by the formula (39).

Rf value: 0.33 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol=4:1)

FAB-MS (m/z): 589 (M+H)⁺

¹ H-NMR (300 MHz, DMSO-d₆), δ(ppm): 10.91 (1H, s), 10.35 (1H, s), 9.95 (1H, s), 8.78 (1H, d, J=8.3 Hz), 8.52 (1H, d, J=8.3 Hz), 7.16 (2H, t, J=7.6 Hz), 6.98-7.06 (3H, m), 5.40 (1H, d, J=5.5 Hz), 5.33 (1H, t, J=5.7 Hz), 5.18 (1H, d, J=5.5 Hz), 4.85 (1H, d, J=4.8 Hz), 4.02 (2H, m), 3.74 (1H, m), 3.53-3.68 (2H, m), 3.30-3.42 (5H, m), 1.97 (4H, m)

Example 38

The compound represented by the formula ##STR57##

90 mg of 6-benzyloxymethyl-1,11-dibenzyloxy-12,13-dehydro-5H-indolo[2,3-a]pyrrolo[3,4-c]carbazole-4,7(6H)-dione, a compound disclosed in PCT/WO91/18003, 1.3 g of silver oxide and 550 mg of 4 Å molecular sieve were suspended in 30 ml of anhydrous benzene. After reflux with heating for 20 minutes, a solution of 416.4 mg of α-bromo-3-deoxy-3-azido-2,4,6-triacetyl-D-glucose in 5 ml of anhydrous benzene was added dropwise over a period of 10 minutes. After further reflux with heating for 2 days, the insoluble matters were filtered using Celite. The filtrate was concentrated to dryness, and the residue was dissolved in 150 ml of ethyl acetate, washed successively with 0.2N hydrochloric acid, water and then saturated saline, dehydrated with anhydrous sodium sulfate, and concentrated to dryness. The residue was dissolved in 5 ml of chloroform, subjected to a chromatograph tower of Sephadex LH-20 (3.0×80 cm) and eluted with chloroform. The fractions containing the desired product were concentrated to dryness, and the residue was purified by preparative thin layer chromatography [n-hexane:acetone:tetrahydrofuran=3:1:0.1 (Rf: 0.5), then toluene:acetone=10:1 (Rf: 0.5)] to give 9.2 mg of 6-benzyloxymethyl-1,11-dibenzyloxy-12,13-dehydro-13-(β-3-deoxy-3-azido-2',4,6-triacetyl-D-glucopyranosyl)-5H-indolo[2,3-a]pyrrolo[3,4-c]carbazole-5,7-(6H)-dione.

9.2 mg of the resultant compound was dissolved in 1 ml of hydrazine monohydrate, and the solution was stirred at room temperature for 4 hours. This was mixed with 30 ml of ethyl acetate, and the mixture was washed successively with 0.2N hydrochloric acid, water and then saturated saline, dehydrated with anhydrous sodium sulfate, and concentrated to dryness. The residue was dissolved in 0.5 ml of tetrahydrofuran--1 ml of methanol, palladium black was added, and the mixture was stirred, under a hydrogen stream, at room temperature for 3 hours. The insoluble matters were filtered using Celite, 1.5 ml of 10% hydrogen chloride-methanol was added to the filtrate, and the mixture was concentrated to dryness. The residue was dissolved in 0.5 ml of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.0×15 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 2.0 mg of the captioned compound represented by the formula (40).

Rf value: 0.5 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; n-butanol:acetic acid: water=4:1:1)

FAB-MS (m/z): 534 (M+H)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 10.80 (1H, s), 10.48 (1H, s), 10.20 (1H, s), 8.79 (1H, d, J=7.9 Hz), 8.52 (3H, br), 8.50 (1H, d, J=9.2 Hz), 7.61 (1H, d, J=6.6 Hz), 7.16 (1H, dd, J=9.2, 9.2 Hz), 7.10 (1H, dd, J=9.2, 9.2 Hz), 7.05 (1H, dd, J=9.2, 9.2 Hz), 7.00 (1H, dd, J=9.2, 9.2 Hz), 6.42 (1H, d, J=5.2 Hz), 6.16 (1H, d, J=3.9 Hz), 5.18 (1H, br), 4.93 (1H, br), 4.40 (1H, m), 4.16 (1H, m), 4.03 (1H, m), 3.78 (1H, m). 3.68 (1H, m), 3.42 (1H, m)

Example 39

The compound represented by the formula ##STR58##

30 mg of the compound obtained in Example A was dissolved in 1.5 ml of N,N-dimethylformamide, 60 mg of cyanoacetohydrazide was added, and the mixture was stirred at 80° C. for 9 hours. This was mixed with 30 ml of ethyl acetate, the mixture was washed successively with water and then saturated saline, and the ethyl acetate layer was dehydrated with anhydrous sodium sulfate and concentrated to dryness. The residue was dissolved in a small quantity of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.5×15 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 27.8 mg of the captioned compound represented by the formula (41).

Rf value: 0.53 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol:tetrahydrofuran=3:1:0.1)

FAB-MS (m/z): 601 (M+H)⁺

¹ H-NMR (300 MHz, DMSO-d₆), δ(ppm): 11.14 (1H, s), 11.01 (1H, s), 10.42 (1H, s), 10.04 (1H, s), 8.65 (1H, d, J=7.6 Hz), 8.47 (1H, d, J=7.6 Hz), 7.21 (2H, t, J=7.6 Hz), 7.05 (3H, t, J=7.6 Hz), 5.41 (2H, d, J=4.5 Hz), 5.19 (1H, d, J=6.8 Hz), 4.90 (1H, d, J=6.8 Hz), 4.13 (2H, s), 4.04 (2H, br), 3.75 (1H, m), 3.64 (2H, m), 3.43 (1H, m)

Example 40

The compound represented by the formula ##STR59##

1 g of 12,13-dihydro-1,11-dihydroxy-13-(β-D-glucopyranosyl)-5H-indolo[2,3-a]pyrrolo[3,4-c]carbazole-5,7(6H)-dione was dissolved in 25 ml of tetrahydrofuran, an ether solution of an excessive quantity of diazomethane was added, the mixture was stirred at 4° C. overnight, and the formed yellow precipitate was collected by filtration. This was dissolved in 3 ml of hydrazine monohydrate, and the solution was subjected to reaction at room temperature for 1.5 hours. After the reaction, 200 ml of purified water was added, and the resultant precipitate was collected by filtration, washed successively with purified water and then methanol, and dried under reduced pressure to give 683.4 mg of the captioned compound represented by the formula (42).

HPLC; Rt, 10.5 minutes (column:Chromatolex ODS, inner diameter 4.6 mm, length 250 mm, detection; UV nm, flow rate; 1 ml/minute, moving phase; methanol:water=6:4)

FAB-MS (m/z): 563 (M+H)⁺

¹ H-NMR (300 MHz, DMSO-d₆) δ(ppm): 10.9 (1H, s), 8.87 (1H, d, J=7.8 Hz), 8.65 (1H, d, J=7.8 Hz), 7.35 (1H, t, J=7.8 Hz), 7.23 (1H, t, J=7.8 Hz), 7.25 (1H, d, J=7.8 Hz), 7.18 (1H, d, J=7.8 Hz), 6.90 (1H, d, J=9.3 Hz), 5.40 (1H, brs), 5.18 (1H, brs), 5.00 (2H, brs), 4.90 (2H, brs), 4.06 (6H, s), 4.00 (2H, m), 3.78 (1H, m), 3.63 (2H, m), 3.42 (1H, m)

Example 41

The compound represented by the formula ##STR60##

708.8 mg of the captioned compound represented by the formula (43) was obtained from 679 mg of the compound obtained in Example 40, according to the process of Example 2.

HPLC; Rt, 10.9 minutes (column:Chromatolex ODS, inner diameter 4.6 mm, length 250 mm, detection; UV nm, flow rate; 1 ml/minute, moving phase; acetonitrile: water=2.8→acetonitrile:water=6:4, 30 minutes linear gradient)

FAB-MS (m/z): 6.18 [M]⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 13.5 (1H, brs), 11.1 (1H, s). 9.01 (1H, s), 8.83 (1H, d, J=7.8 Hz), 8.63 (1H, d, J=7.8 Hz), 7.39 (1H, t, J=7.8 Hz), 7.37 (1H, t, J=7.8 Hz), 7.29 (1H, d, J=7.8 Hz), 7.22 (1.H, d, J=7.8 Hz), 6.94 (1H, d, J=9.3 Hz), 5.43 (1H, d, J=5.4 Hz), 5.22 (1H, d, J=5.4 Hz), 5.01 (1H, brs), 4.93 (1H, d, J=5.4 Hz), 4.07 (6H, s), 4.05 (1H, m), 3.96 (1H, m), 3.79 (1H, m), 3.60 (2H, m), 3.44 (1H, m)

Example 42

The compound represented by the formula ##STR61##

704 mg of the compound obtained in Example 41 was dissolved in 10 ml of N,N-dimethylformamide, 60 mg of palladium-carbon (Pd-C) was added, and the mixture was subjected to hydrogenation at room temperature for 6 hours under stirring. The reaction mixture was filtered using a sheet of filter paper on which Celite was spread to remove Pd-C, 200 ml of ethyl acetate was added to the filtrate, and the mixture was extracted with 50 ml of sodium bicarbonate aqueous solution (pH 8). The water layer was adjusted to pH 2 and extracted with ethyl acetate (500 ml). The ethyl acetate layer was extracted with 2% sodium bicarbonate aqueous solution (70 ml). The 2% sodium bicarbonate aqueous solution layer was concentrated under reduced pressure, adsorbed on a column of Diaion HP 20 (inner diameter 3 cm, length 30 cm), washed with water, and then eluted with 300 ml of methanol. The methanol eluate was concentrated to dryness, the residue was dissolved in a small quantity of N,N-dimethylformamide, and the solution was subjected to preparative HPLC (column: Chromatolex ODS, inner diameter 20 mm, length 250 mm, detection; UV 310 nm, flow rate; 9 ml/minute, moving phase; acetonitrile:water=25:75). The fractions containing the desired product were concentrated to dryness, and the residue was dissolved in a small quantity of water, subjected to column chromatography of Sephadex G-15 (inner diameter 3 cm, length 63 cm) and eluted with water:methanol=9:1. The fractions containing the desired product were concentrated and then freeze dried to give 84.2 mg of the sodium salt of the captioned compound represented by the formula (44).

HPLC; Rt, 8.9 minutes (column: Chromatolex ODS, inner diameter 4.6 mm, length 250 mm, detection; UV 310 nm, flow rate; 1 ml/minute, moving phase; acetonitrile: water:trifluoroacetic acid=300:700:1)

FAB-MS (m/z): 643 (M+Na)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 10.9 (1H, brs), 8.85 (1H, d, J=7.8 Hz), 8.63 (1H, d, J=7.8 Hz), 7.33 (1H, t, J=7.8 Hz), 7.31 (1H, t, J=7.8 Hz), 7.24 (1H, d, J=7.8 Hz), 7.16 (1H, d, J=7.8 Hz), 6.89 (1H, d, J=9.3 Hz), 5.63 (1H, brs), 5.42 (1H, brs), 5.10 (1H, brs), 4.99 (1H, brs), 4.06 (6H, s), 4.02 (2H, m), 3.80 (1H, m), 3.67 (1H, t, J=8.8 Hz), 3.58 (1H, m), 3.42 (1H, t, J=8.3 Hz), 3.34 (2H, s)

Example 43

The compound represented by the formula ##STR62##

23.8 mg of the captioned compound represented by the formula (45) was obtained from 70 mg of the compound obtained in Example 4, according to the same process as in Example 2.

FAB-MS (m/z): 641 (M+H)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 10.8 (1H, s), 9.26 (1H, d, J=7.8 Hz), 9.09 (1H, d, J=7.8 Hz), 8.94 (1H, s), 7.78 (1H, d, J=7.8 Hz), 7.74 (1H, d, J=7.8 Hz), 7.50 (2H, t, J=7.8 Hz), 6.98 (1H, d, J=9.3 Hz), 5.44 (1H, d, J=5.9 Hz), 5.33 (1H, brs), 5.09 (1H, d, J=5.4 Hz), 3.96 (2H, m), 3.85 (1H, m), 3.67 (2H, m), 3.59 (3H, s), 3.56 (1H, m)

Example 44

The compound represented by the formula ##STR63##

210 mg of the captioned compound represented by the formula (46) was obtained from 1 g of the compound obtained in Example 43, according to the same process as in Example 42.

FAB-MS (m/z): 643 (M+H)⁺

¹ H-NMR (500 MHz, DMSO-d₆), δ(ppm): 10.7 (1H, s), 9.26 (1H, d, J=7.8 Hz), 9.09 (1H, d, J=7.8 Hz), 7.74 (1H, d, J=7.8 Hz), 7.71 (1H, d, J=7.8 Hz), 7.46 (2H, t, J=7.8 Hz), 6.93 (1H, d, J=9.2 Hz), 6.00 (1H, brs), 5.42 (1H, brs), 5.31 (1H, brs), 5.03 (1H, brs), 3.96 (2H, brs), 3.85 (2H, s), 3.83 (1H, m), 3.59 (3H, s), 3.50-3.70 (3H, m)

Example 45

The compound represented by the formula ##STR64##

48.2 mg of the captioned compound represented by the formula (47) was obtained from 51.4 mg of the compound obtained in Example 4, according to the same process as in Example 5.

FAB-MS (m/z): 613 (M+H)⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 10.9 (1H, brs), 10.8 (1H, brs), 9.20 (1H, m), 9.03 (1H, m), 8.48 (1H, s), 7.75 (1H, d, J=7.8 Hz), 7.70 (1H, d, J=7.8 Hz), 7.45 (2H, t, J=7.8 Hz), 6.93 (1H, brt, J=9.3 Hz), 5.41 (2H, m), 5.04 (1H, d, J=5.9 Hz), 3.99 (2H, brs), 3.86 (1H, m), 3.60 (3H, s), 3.52-3.67 (3H, m)

Example 46

The compound represented by the formula ##STR65##

13 mg of the captioned compound represented by the formula (48) was obtained from 14.1 mg of the compound obtained in Example 4, according to the same process as in Example 6.

FAB-MS (m/z): 627 (M+H)⁺

¹ H-NMR (500MHz, DMSO-d₆), δ(ppm): 10.8 (2H, s), 9.20 (1H, m), 9.04 (1H, m), 7.74 (2H, m), 7.47 (2H, m), 6.93 (1H, m), 5.41 (1H, m), 5.32 (1H, brs), 5.04 (1H, m), 3.96 (2H, brs), 3.85 (1H, m), 3.58 (3H, s), 3.50-3.70 (3H, m), 2.12 (3H, s)

Example 47

The compound represented by the formula ##STR66##

1 ml of hydrazine monohydrate was added to 3.2 mg of 12,13-dihydro-2,10-dihydroxy-13-(β-D-glucopyranosyl)-5H-indolo[2,3-a]pyrrolo[3,4-c]carbazole-5,7(6H)-dione, and the mixture was stirred at room temperature for 2 hours. This was distributed with ethyl acetate-0.2N hydrochloric acid, and the ethyl acetate layer was washed successively with water then saturated saline, and concentrated to dryness. The residue was dissolved in a small quantity of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.0×5 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 3.0 mg of the captioned compound represented by the formula (49).

Rf value: 0.22 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol:tetrahydrofuran=3:1:1)

FAB-MS (m/z): 534 [M]⁺

¹ H-NMR (300 MHz, DMSO-d₆), δ(ppm): 11.16 (1H, s), 9.76 (1H, s), 9.73 (1H, s), 8.90 (1H, d, J=7.3 Hz), 8.82 (1H, d, J=7.3 Hz), 7.18 (1H, d, J=2.0 Hz), 6.98 (1H, d, J=2.0 Hz), 6.83 (2H, dr, J=2.0, 7.3 Hz), 5.97 (1H, d, J=7.2 Hz), 5.84 (1H, t, J=3.3 Hz), 5.32 (1H, d, J=5.3 Hz), 5.10 (1H, d, J=5.3 Hz), 4.93 (1H, d, J=5.2 Hz), 4.90 (2H, s), 4.04-3.86 (2H, m), 3.78 (1H, m), 3.60-3.35 (3H, m)

Example 48

The compound represented by the formula ##STR67##

0.4 ml of hydrazine hydrate was added to 7.1 mg of 2,10-difluoro-12,13-dihydro-13-(β-D-glucopyranosyl)-5H-indolo[2,3-a]pyrrolo[3,4-c]carbazole-5,7(6H)-dione, and the mixture was stirred at room temperature for 40 minutes. 1.34 ml of concentrated hydrochloric acid was added thereto, and the mixture was extracted with ethyl acetate. The ethyl acetate layer was washed with water and concentrated. The residue was dissolved in 3.7 ml of N,N-dimethylformamide and 0.37 ml of concentrated hydrochloric acid, and the solution was stirred at room temperature overnight. This was distributed between ethyl acetate and water, and the ethyl acetate layer was concentrated to dryness. The residue was dissolved in a small quantity of ethanol, subjected to a chromatograph tower of Sephadex LH-20 and eluted with ethanol. The fractions containing the desired product were concentrated to dryness to give 4.6 mg of the captioned compound represented by the formula (50).

FAB-MS (m/z): 566 [M]⁺

¹ H-NMR (400 MHz, DMSO-d₆), δ(ppm): 11.9 (1H, s), 10.8 (1H, brs), 9.07 (1H, dd, J=5.8, 8.8 Hz), 9.01 (1H, dd, J=5.9, 8.8 Hz), 8.45 (1H, s), 7.93 (1H, brd, J=8.8 Hz), 7.44 (1H, brd, J=8.8 Hz), 7.27 (2H, m), 6.28 (1H, d, J=8.8 Hz), 6.20 (1H, brs), 5.42 (1H, brs), 5.13 (1H, brd. J=5.4 Hz), 4.96 (1H, d, J=5.4 Hz), 4.09 (1H, brd, J=7.3 Hz), 3.94 (2H, m), 3.83 (1H, brd, J=7.3 Hz), 3.58 (1H, m), 3.45 (1H, m)

Example 49

The compound represented by the formula ##STR68## wherein Bn represents a benzyl group.

100 mg of 6-benzyloxymethyl-1,11-dibenzyloxy-12,13-dihydro-5H-indolo[2,3-a]pyrrolo[3,4-c]carbazole-5,7(6H)-dione, 1.4 g of silver oxide and 0.7 g of 4 Å molecular sieve were suspended in 40 ml of anhydrous benzene, the suspension was refluxed with heating for 20 minutes, and then a solution of 1-bromo-2,3,5-tri-O-acetyl-D-ribose in 10 ml of anhydrous benzene was added dropwise over a period of 10 minutes. The mixture was further refluxed with heating for 3 hours, and the insoluble matters were filtered using Celite.

The filtrate was concentrated to dryness, and the residue was dissolved in 100 ml of ethyl acetate and the solution was washed successively with 0.2N hydrochloric acid, water and then saturated saline, dried over anhydrous sodium sulfate, and concentrated to dryness. The residue was dissolved in chloroform, subjected to a chromatograph tower of Sephadex LH-20 (2.5×20 cm) and eluted with chloroform. The fractions containing the desired product were concentrated to dryness, the residue was subjected to a chromatograph tower of silica gel (2.5×25 cm) and eluted with toluene-ethyl acetate (3:1), and the fractions containing the desired product were concentrated to dryness. The residue was further purified by preparative thin layer chromatography (toluene-ethyl acetate=5:1 (Rf=0.6)) to give 20.8 mg of 6-benzyloxymethyl-1,11-dibenzyloxy-12,13-dihydro-13-(β-2,3,5-tri-o-acetyl-D-ribofuranosyl)-5H-indolo[2,3-a]pyrrolo[3,4-c]carbazole-5,7(6H)-dione

20.8 mg of this compound was dissolved in 2 ml of hydrazine monohydrate, and the solution was stirred at room temperature for 2 hours. This was mixed with 30 ml of ethyl acetate, the mixture was washed successively with 0.2N hydrochloric acid, water and then saturated saline, and concentrated to dryness. The residue was dissolved in methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.0×15 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness, and the residue was purified by preparative thin layer chromatography (chloroform-methanol=10:1 (Rf=0.5)) to give 2.9 mg of the captioned compound represented by the formula (51).

Rf value: 0.5 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol=10:1)

FAB-MS (m/z): 684 [M]⁺

¹ H-NMR (300 MHz, DMSO-d₆), δ(ppm): 10.45 (1H, s), 8.90 (1H, d, J=0.75 Hz), 8.68 (1H, d, J=0.75 Hz), 7.18 (2H, d, J=0.75 Hz), 7.11 (2H, d, J=0.75 Hz), 7.20-7.50 (11H, m), 5.35-5.45 (5H, m), 5.17 (1H, d, J=0.38 Hz), 5.10 (1H, d, J=0.45 Hz), 4.98 (2H, s), 3.90-4.00 (2H, m), 3.60-3.70 (2H, m)

Example 50

The compound represented by the formula ##STR69##

33.0 mg of the compound obtained in Example A was dissolved in 3 ml of N,N-dimethylformamide, 8.4 mg of hydroxyacetohydrazide was added, and the mixture was stirred at 80° C. for 2 days. This was concentrated to dryness, and the residue was dissolved in a small quantity of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.5×25 cm), and eluted with methanol. The fractions containing the desired product were concentrated to dryness, and the residue was dissolved in 30 ml of ethyl acetate. The solution was washed with water, and the ethyl acetate layer was dried over anhydrous sodium sulfate and concentrated to dryness. The residue was dissolved in a small quantity of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.5×15 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 29.0 mg of the captioned compound represented by the formula (52).

FAB-MS (m/z): 593 [M+H]⁺

¹ H-NMR (300 MHz, DMSO-d₆), δ(ppm): 11.00 (1H, s), 10.55 (1H, s), 10.41 (1H, s), 10.02 (1H, s), 8.63 (1H, d, J=7.8 Hz), 8.47 (1H, d, J=7.8 Hz), 7.20 (2H, t, J=7.8 Hz), 7.04 (3H, m), 5.88 (1H, t, J=7.0 Hz), 5.41 (1H, d, J=6.2 Hz), 5.35 (1H, br), 5.20 (1H, d, J=6.2 Hz), 4.90 (1H, d, J=6.2 Hz), 4.16 (2H, d, J=5.7 Hz), 4.03 (2H, m), 3.74 (1H, m), 3.59-3.68 (2H, m), 3.39 (1H, m)

Example 51

The compound represented by the formula ##STR70##

35.0 mg of the compound obtained in Example A was dissolved in 1.0 ml of N,N-dimethylformamide, 35.0 mg of ethylhydrazine oxalate and 0.5 ml of saturated sodium bicarbonate aqueous solution were added, and the mixture was stirred at 80° C. for 1 day. This was concentrated to dryness, and the residue was dissolved in a small quantity Of methanol, subjected to a chromatograph tower of Sephadex LH-20 (1.5×15 cm) and eluted with methanol. The fractions containing the desired product were concentrated to dryness to give 20.8 mg of the captioned compound represented by the formula (53).

Rf value: 0.5 (produced by Merck Co., Kiesel gel 60F₂₅₄, developing solvent; chloroform:methanol:tetrahydrofuran=2:1:1)

FAB-MS (m/z): 563 [M+H]⁺

¹ H-NMR (300 MHz, DMSO-d₆), δ(ppm): 10.90 (1H, s), 10.35 (1H, s), 9.96 (1H, s), 8.72 (1H, d, J=7.9 Hz), 8.54 (1H, d, J=7.9 Hz), 7.17 (2H, t, J=7.9 Hz), 7.03 (3H, m), 5.72 (1H, t, J=4.8 Hz), 5.41 (1H, d, J=6.3 Hz), 5.35 (1H, t, J=4.0 Hz), 5.21 (1H, d, J=4.0 Hz), 4.87 (1H, d, J=6.3 Hz), 3.96-4.09 (2H, m), 3.73-3.77 (1H, m), 3.58-3.67 (2H, m), 3.37-3.45 (1H, m), 3.07 (2H, m), 1.09 (3H, t, J=7.1 Hz)

Example 52

50 g of the compound of Example 5 was dissolved in a solution wherein 600 g of macrogol 400 of the Japanese Pharmacopoeia was dissolved in 400 g of distilled water for injection, and the solution was filtered for removal of bacteria using a filter of 0.2 μm. 5 ml portions of the filtrate were filled into washed and sterilized vials according to a conventional method, and the vials were stopped and capped to give an injection containing 250 mg of the compound of Example 5 per vial. Administration is made using an agent for intravenous drip wherein 5 to 10 ml of this injection (250 to 500 mg of the compound of Example 5) was added to and diluted with 500 ml of an infusion such as 5% glucose. 

We claim:
 1. A process for preparation of a compound of the formula (VII) ##STR71## wherein X¹ and X² are each a hydroxyl group, which comprises culturing a microorganism selected from the group consisting of Microtetraspora sp. A34549, Saccharothrix aerocolonigenes ATCC 39243, and a mutant thereof, all of which have ability to glycosylate a compound represented by the formula ##STR72## wherein X¹ and X² have the same meanings as defined above, in a nutrient medium containing the compound of formula (VIII), and recovering the compound represented by formula (VII) from the culture medium.
 2. The process according to claim 1, wherein the microorganism is Microtetraspora sp. A34549.
 3. The process according to claim 1, wherein the microorganism is Saccharothrix aerocolonigenes strain ATCC
 39243. 4. The process for preparation according to claim 1 wherein the group represented by ##STR73## is a glucose residue.
 5. A process for preparation of a compound of the formula (VII) ##STR74## wherein X¹ and X² each represent a hydroxyl group, which comprises culturing a microorganism selected from the group consisting of Microtetraspora sp. A34549, and Saccharothrix aerocolonigenes ATCC 39243, and a mutant of either of said microorganisms, all of which have ability to glycosylate a compound represented by the formula ##STR75## wherein X¹ and X² have the same meanings as defined above, in a nutrient medium, adding the compound of formula (VIII) to the nutrient medium containing growing cells, and recovering the compound of formula (VII) from the culture medium.
 6. The process of claim 5 wherein the Microtetraspora sp. A34549 is cultured.
 7. The process of claim 5 wherein the Saccharothrix aerocolonigenes ATCC 39243 is cultured. 